Artículos de revistas
Acute Exercise Improves Insulin Clearance And Increases The Expression Of Insulin-degrading Enzyme In The Liver And Skeletal Muscle Of Swiss Mice
Registro en:
Plos One. Public Library Science, v. 11, p. , 2016.
1932-6203
WOS:000381516100136
10.1371/journal.pone.0160239
Autor
Kurauti
Mirian A.; Freitas-Dias
Ricardo; Ferreira
Sandra M.; Vettorazzi
Jean F.; Nardelli
Tarlliza R.; Araujo
Hygor N.; Santos
Gustavo J.; Carneiro
Everardo M.; Boschero
Antonio C.; Rezende
Luiz F.; Costa-Junior
Jose M.
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The effects of exercise on insulin clearance and IDE expression are not yet fully elucidated. Here, we have explored the effect of acute exercise on insulin clearance and IDE expression in lean mice. Male Swiss mice were subjected to a single bout of exercise on a speed/angle controlled treadmill for 3-h at approximately 60-70% of maximum oxygen consumption. As expected, acute exercise reduced glycemia and insulinemia, and increased insulin tolerance. The activity of AMPK-ACC, but not of IR-Akt, pathway was increased in the liver and skeletal muscle of trained mice. In an apparent contrast to the reduced insulinemia, glucose-stimulated insulin secretion was increased in isolated islets of these mice. However, insulin clearance was increased after acute exercise and was accompanied by increased expression of the insulin-degrading enzyme (IDE), in the liver and skeletal muscle. Finally, C2C12, but not HEPG2 cells, incubated at different concentrations of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside (AICAR) for 3-h, showed increased expression of IDE. In conclusion, acute exercise increases insulin clearance, probably due to an augmentation of IDE expression in the liver and skeletal muscle. The elevated IDE expression, in the skeletal muscle, seems to be mediated by activation of AMPK-ACC pathway, in response to exercise. We believe that the increase in the IDE expression, comprise a safety measure to maintain glycemia at or close to physiological levels, turning physical exercise more effective and safe. 11 7 Fundacao de Amparo e Pesquisa do Estado de Sao Paulo (FAPESP) [2013/07607-8, 2014/24719-7] Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)