Artículos de revistas
Multimodal Evaluation Of Ultra-short Laser Pulses Treatment For Skin Burn Injuries
Registro en:
Biomedical Optics Express. Optical Soc Amer, v. 8, p. 1575 - 1588, 2017.
2156-7085
WOS:000395942600024
10.1364/BOE.8.001575
Autor
dos Santos
Moises Oliveira; Latrive
Anne; Augusto de Castro
Pedro Arthur; de Rossi
Wagner; Tenorio Zorn
Telma Maria; Samad
Ricardo Elgul; Freitas
Anderson Zanardi; Cesar
Carlos Lenz; Vieira Junior
Nilson Dias; Zezell
Denise Maria
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Thousands of people die every year from burn injuries. The aim of this study is to evaluate the feasibility of high intensity femtosecond lasers as an auxiliary treatment of skin burns. We used an in vivo animal model and monitored the healing process using 4 different imaging modalities: histology, Optical Coherence Tomography (OCT), Second Harmonic Generation (SHG), and Fourier Transform Infrared (FTIR) spectroscopy. 3 dorsal areas of 20 anesthetized Wistar rats were burned by water vapor exposure and subsequently treated either by classical surgical debridement, by laser ablation, or left without treatment. Skin burn tissues were non-invasively characterized by OCT images and biopsied for further histopathology analysis, SHG imaging and FTIR spectroscopy at 3, 5, 7 and 14 days after burn. The laser protocol was found as efficient as the classical treatment for promoting the healing process. The study concludes to the validation of femtosecond ultra-short pulses laser treatment for skinburns, with the advantage of minimizing operatory trauma. (C) 2017 Optical Society of America 8 3 1575 1588 FAPESP/CEPID [05/51689-2, 11/51959-0] CNPq/INCT [573.916/2008-0, 573913/2008-00] CNPq/PQ [312397/2013-5] CAPES/PROCAD [8881.068505/2014-01] FAPEAM Programa RH-POSGRAD Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)