Artículos de revistas
Variation In Olfactory Neuron Repertoires Is Genetically Controlled And Environmentally Modulated
Registro en:
Elife. Elife Sciences Publications Ltd, v. 6, p. , 2017.
2050-084X
WOS:000400014600001
10.7554/eLife.21476
Autor
Ibarra-Soria
Ximena; Nakahara
Thiago S.; Lilue
Jingtao; Jiang
Yue; Trimmer
Casey; Souza
Mateus A. A.; Netto
Paulo H. M.; Ikegami
Kentaro; Murphy
Nicolle R.; Kusma
Mairi; Kirton
Andrea; Saraiva
Luis R.; Keane
Thomas M.; Matsunami
Hiroaki; Mainland
Joel; Papes
Fabio; Logan
Darren W.
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The mouse olfactory sensory neuron (OSN) repertoire is composed of 10 million cells and each expresses one olfactory receptor (OR) gene from a pool of over 1000. Thus, the nose is sub-stratified into more than a thousand OSN subtypes. Here, we employ and validate an RNA-sequencing-based method to quantify the abundance of all OSN subtypes in parallel, and investigate the genetic and environmental factors that contribute to neuronal diversity. We find that the OSN subtype distribution is stereotyped in genetically identical mice, but varies extensively between different strains. Further, we identify cis-acting genetic variation as the greatest component influencing OSN composition and demonstrate independence from OR function. However, we show that olfactory stimulation with particular odorants results in modulation of dozens of OSN subtypes in a subtle but reproducible, specific and time-dependent manner. Together, these mechanisms generate a highly individualized olfactory sensory system by promoting neuronal diversity. 6 Wellcome [098051] Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [09/00473-0, 2015/50371-0] European Molecular Biology Organization Young Investigator Award Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)