Artículos de revistas
15d-pgj(2)-loaded Solid Lipid Nanoparticles: Physicochemical Characterization And Evaluation Of Pharmacological Effects On Inflammation
Registro en:
Plos One. Public Library Science, v. 11, p. , 2016.
1932-6203
WOS:000382877200038
10.1371/journal.pone.0161796
Autor
Silva de Melo
Nathalie Ferreira; de Macedo
Cristina Gomes; Bonfante
Ricardo; Abdalla
Henrique Ballassini; Goncalves da Silva
Camila Morais; Pasquoto
Tatiane; de Lima
Renata; Fraceto
Leonardo Fernandes; Clemente-Napimoga
Juliana Trindade; Napimoga
Marcelo Henrique
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), a peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist, has physiological properties including pronounced anti-inflammatory activity, though it binds strongly to serum albumin. The use of solid lipid nanoparticles (SLN) can improve therapeutic properties increasing drug efficiency and availability. 15d-PGJ(2)-SLN was therefore developed and investigated in terms of its immunomodulatory potential. 15d-PGJ(2)-SLN and unloaded SLN were physicochemically characterized and experiments in vivo were performed. Animals were pretreated with 15d-PGJ(2)-SLN at concentrations of 3, 10 or 30 mu g.kg(-1) before inflammatory stimulus with carrageenan (Cg), lipopolysaccharide (LPS) or mBSA (immune response). Interleukins (IL-1 beta, IL-10 and IL-17) levels were also evaluated in exudates. The 15d-PGJ(2)-SLN system showed good colloidal parameters and encapsulation efficiency of 96%. The results showed that the formulation was stable for up to 120 days with low hemolytic effects. The 15d-PGJ(2)-SLN formulation was able to reduce neutrophil migration in three inflammation models tested using low concentrations of 15d-PGJ(2). Additionally, 15d-PGJ(2)-SLN increased IL-10 levels and reduced IL-1 beta as well as IL-17 in peritoneal fluid. The new 15d-PGJ(2)-SLN formulation highlights perspectives of a potent anti-inflammatory system using low concentrations of 15d-PGJ(2). 11 8 Brazilian funding agency Sao Paulo Research Foundation (FAPESP) Brazilian funding agency National Council for Scientific and Technological Development (CNPq) Sao Paulo Research Foundation [2014/11016-8] CNPq [303555/2013-0] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)