Maldi Mass Spectrometry Reveals That Cumulus Cells Modulate The Lipid Profile Of In Vitro-matured Bovine Oocytes

dc.creatorVireque
dc.creatorAlessandra A.; Tata
dc.creatorAlessandra; Belaz
dc.creatorKatia Roberta A.; Grazia
dc.creatorJoao Gabriel V.; Santos
dc.creatorFabio N.; Arnold
dc.creatorDaniel R.; Basso
dc.creatorAndrea C.; EBerlin
dc.creatorMarcos N.; Silva-de-Sa
dc.creatorMarcos Felipe; Ferriani
dc.creatorRui A.; Sa Rosa-e-Silva
dc.creatorAna Carolina J.
dc.date2017
dc.date2017-11-13T13:43:20Z
dc.date2017-11-13T13:43:20Z
dc.date.accessioned2018-03-29T05:58:05Z
dc.date.available2018-03-29T05:58:05Z
dc.identifierSystems Biology In Reproductive Medicine. Taylor & Francis Inc, v. 63, p. 86 - 99, 2017.
dc.identifier1939-6368
dc.identifier1939-6376
dc.identifierWOS:000396840500003
dc.identifier10.1080/19396368.2017.1289279
dc.identifierhttp://www.tandfonline.com/doi/abs/10.1080/19396368.2017.1289279?journalCode=iaan20
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328536
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1365561
dc.descriptionThe influence of cumulus cells (CC) on the lipid profile of bovine oocytes matured in two different lipid sources was investigated. Cumulus-oocyte complexes (COC) or denuded oocytes (DO) were matured in tissue culture medium (TCM) supplemented with fetal bovine serum (FBS) or serum substitute supplement (SSS). Lipid profiles of TCM, serum supplements, immature CC and oocyte (IO), and in vitro-matured oocytes from COC and DO were then analyzed by matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and submitted to partial least squaresdiscriminant analysis (PLS-DA). The developmental competence of such oocytes was also assessed. Differences in lipid composition were observed between two types of sera and distinctly influenced the lipid profile of CC. As revealed by PLS-DA, the abundance of specific ions corresponding to triacylglycerols (TAG) or phospholipids (PL) were higher in COC compared to DO both supplemented with FBS or SSS and to some extent affected the subsequent DO in vitro embryo development. DO exposed to SSS had however a marked diminished ability to develop to the blastocyst stage. These results indicate a modulation by CC of the oocyte TAG and PL profiles associated with a specific cell response to the serum supplement used for in vitro maturation.
dc.description63
dc.description2
dc.description86
dc.description99
dc.languageEnglish
dc.publisherTaylor & Francis Inc
dc.publisherPhiladelphia
dc.relationSystems Biology in Reproductive Medicine
dc.rightsfechado
dc.sourceWOS
dc.subjectFetal Bovine Serum
dc.subjectIn Vitro Maturation
dc.subjectLipidomics
dc.subjectSerum
dc.subjectSubstitute
dc.subjectSupplement
dc.titleMaldi Mass Spectrometry Reveals That Cumulus Cells Modulate The Lipid Profile Of In Vitro-matured Bovine Oocytes
dc.typeArtículos de revistas


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