Artículos de revistas
Tolerogenic Vaccination With Mog/vitd Overcomes Aggravating Effect Of C-albicans In Experimental Encephalomyelitis
Registro en:
Cns Neuroscience & Therapeutics. Wiley-blackwell, v. 22, p. 807 - 816, 2016.
1755-5930
1755-5949
WOS:000383628000001
10.1111/cns.12572
Autor
Fraga-Silva
Thais F. C.; Mimura
Luiza A. N.; Zorzella-Pezavento
Sofia F. G.; Ishikawa
Larissa L. W.; Franca
Thais G. D.; Thome
Rodolfo; Verinaud
Liana; Arruda
Maria S. P.; Sartori
Alexandrina
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Multiple sclerosis (MS) is an immune-mediated demyelinating disorder of the central nervous system (CNS). We described that Candida albicans (Ca) aggravates experimental autoimmune encephalomyelitis (EAE) that is a model to study MS. We also observed that vaccination with a myelin peptide (MOG) in the presence of vitamin D (VitD) protected mice against EAE. In this work, we investigated whether Ca infection interferes with the efficacy of this vaccine. MethodsEAE was induced in C57BL/6 female mice previously vaccinated with MOG+VitD and then infected 3 days before encephalomyelitis induction. ResultsVaccination was able to control EAE development in infected mice. These animals gained weight, and only a few progressed to very low clinical scores. Protection was confirmed by a lower inflammatory infiltration in the CNS and was also associated with a reduced production of encephalitogenic cytokines by spleen and CNS cell cultures. The elevated percentage of CD25(+)FoxP3(+) cells suggests that regulatory T cells are involved in the protection. Adoptive transfer of splenocytes from mice vaccinated with MOG+VitD supports the view that protection is mediated by immunoregulatory cells. ConclusionTogether, these experiments provide evidence demonstrating that EAE can be prevented by the inverse vaccination with MOG+VitD even in the presence of a disease-aggravating infectious agent. 22 10 807 816 Sao Paulo Research Foundation (FAPESP) [2012/12540-7] FAPESP [2013/ 14353-2] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)