Artículos de revistas
Dendritic Cells Stimulated By Cationic Liposomes
Registro en:
Journal Of Nanoscience And Nanotechnology. Amer Scientific Publishers, v. 16, p. 270 - 279, 2016.
1533-4880
1533-4899
WOS:000369680400025
10.1166/jnn.2016.10714
Autor
Vitor
Micaela Tamara; Bergami-Santos
Patricia Cruz; Piedade Cruz
Karen Steponavicius; Pinho
Mariana Pereira; Marzagao Barbuto
Jose Alexandre; De La Torre
Lucimara Gaziola
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Immunotherapy of cancer aims to harness the immune system to detect and destroy cancer cells. To induce an immune response against cancer, activated dendritic cells (DCs) must present tumor antigens to T lymphocytes of patients. However, cancer patients' DCs are frequently defective, therefore, they are prone to induce rather tolerance than immune responses. In this context, loading tumor antigens into DCs and, at the same time, activating these cells, is a tempting goal within the field. Thus, we investigated the effects of cationic liposomes on the DCs differentiation/maturation, evaluating their surface phenotype and ability to stimulate T lymphocytes proliferation in vitro. The cationic liposomes composed by egg phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium propane and 1,2-dioleoylphosphatidylethanolamine (50/25/25% molar) were prepared by the thin film method followed by extrusion (65 nm, polydispersity of 0.13) and by the dehydration rehydration method (95% of the population 107 nm, polydispersity of 0.52). The phenotypic analysis of dendritic cells and the analysis of T lymphocyte proliferation were performed by flow cytometry and showed that both cationic liposomes were incorporated and activated dendritic cells. Extruded liposomes were better incorporated and induced higher CD86. expression for dendritic cells than dehydrated rehydrated vesicles. Furthermore, dendritic cells which internalized extruded liposomes also provided stronger T lymphocyte stimulation. Thus, cationic liposomes with a smaller size and polydispersity seem to be better incorporated by dendritic cells. Hence, these cationic liposomes could be used as a potential tool in further cancer immunotherapy strategies and contribute to new strategies in immunotherapy. 16 1 270 279 Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)