Artículos de revistas
Layer-by-layer Assembly Of Functionalized Reduced Graphene Oxide For Direct Electrochemistry And Glucose Detection
Registro en:
Materials Science And Engineering: C-materials For Biological Applications. Elsevier Science Bv, v. 68, p. 739 - 745, 2016.
0928-4931
1873-0191
WOS:000382600000087
10.1016/j.msec.2016.06.001
Autor
Tavares Mascagni
Daniela Branco; Miyazaki
Celina Massumi; da Cruz
Nilson Cristino; de Moraes
Marli Leite; Riul
Antonio
Jr.; Ferreira
Marystela
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) We report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. The electron transport properties of graphene were combined with the specificity provided by the enzyme. rGO was obtained and functionalized using chemical methods, being positively charged with poly(diallyldimethylammonium chloride) to form GPDDA, and negatively charged with poly(styrene sulfonate) to form GPSS. Stable aqueous dispersions of GPDDA and GPSS are easily obtained, enabling the growth of LbL films on various solid supports. The use of graphene in the immobilization of GOx promoted Direct Electron Transfer, which was evaluated by Cyclic Volt-ammetry. Amperometric measurements indicated a detection limit of 13.4 mu mol.L-1 and sensitivity of 2.47 mu A.cm(-2). mmol(-1).L for glucose with the (GPDDA/GPSS)(1)/(GPDDA/GOx)(2) architecture, whose thickness was 19.80 +/- 0.28 nm, as determined by Surface Plasmon Resonance (SPR). The sensor may be useful for clinical analysis since glucose could be detected even in the presence of typical interfering agents and in real samples of a lactose-free milk and an electrolyte solution to prevent dehydration. (C) 2016 Elsevier B.V. All rights reserved. 68 739 745 FAPESP [2014/17519-1, 2014/15093-7] CNPq CAPES (Brazil) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)