Artículos de revistas
Epithelial Ovarian Cancer Diagnosis Of Second-harmonic Generation Images: A Semiautomatic Collagen Fibers Quantification Protocol
Registro en:
Cancer Informatics. Sage Publications Ltd, v. 16, p. , 2017.
1176-9351
WOS:000397646200001
10.1177/1176935117690162
Autor
Zeitoune
Angel A.; Luna
Johana S. J.; Sanchez Salas
Kynthia; Erbes
Luciana; Cesar
Carlos L.; Andrade
Liliana A. L. A.; Carvahlo
Hernades F.; Bottcher-Luiz
Fatima; Casco
Victor H.; Adur
Javier
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) A vast number of human pathologic conditions are directly or indirectly related to tissular collagen structure remodeling. The nonlinear optical microscopy second-harmonic generation has become a powerful tool for imaging biological tissues with anisotropic hyperpolarized structures, such as collagen. During the past years, several quantification methods to analyze and evaluate these images have been developed. However, automated or semiautomated solutions are necessary to ensure objectivity and reproducibility of such analysis. This work describes automation and improvement methods for calculating the anisotropy (using fast Fourier transform analysis and the gray-level co-occurrence matrix). These were applied to analyze biopsy samples of human ovarian epithelial cancer at different stages of malignancy (mucinous, serous, mixed, and endometrial subtypes). The semiautomation procedure enabled us to design a diagnostic protocol that recognizes between healthy and pathologic tissues, as well as between different tumor types. 16 CEPOF (Optics and Photonics Research Center, FAPESP) INFABIC (National Institute of Photonics Applied to Cell Biology, FAPESP) INFABIC (National Institute of Photonics Applied to Cell Biology, CNPq) PIO CONICET-UNER [4337/15, 14620140100004 CO] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)