Artículos de revistas
Antimicrobial Photodynamic Effect Of Extracts And Oxoaporphine Alkaloid Isomoschatoline From Guatteria Blepharophylla
Registro en:
Journal Of Photochemistry And Photobiology And B-biology. Elsevier Science Sa, v. 160, p. 154 - 162, 2016.
1011-1344
WOS:000378453500019
10.1016/j.jphotobiol.2016.04.010
Autor
Andreazza
Nathalia Luiza; de Lourenco
Caroline Caramano; Hernandez-Tasco
Alvaro Jose; Pinheiro
Maria Lucia B.; Alves Stefanello
Maria Elida; Costa
Emmanoel Vilaca; Salvador
Marcos Jose
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Photodynamic Therapy, a tumor therapy idealized at the beginning of the last century, emerges nowadays as a promising treatment alternative against infectious diseases. In this study we report a bioguided study of Guatteria blepharophylla phytoderivatives for antimicrobial PDT. Crude extracts and fraction from the species bark were obtained and further fractionated for substances isolation. All samples were evaluated in relation to their photophysical (absorbance and fluorescence) and photochemical properties (1,3-DPBF bleaching method). Then, bioassays were conducted using as biological models bacteria and yeast strains and a diode laser as a light source. Phytochemical analyses lead to the isolation of 5 isoquinoline alkaloids from oxoaporphine subclass, denominated GB1 to GB5. Photophysical and photochemical analysis showed that extracts, fraction and GB1 (isomoschatoline) presented absorption profile with bands at 600-700 nm and were positive for singlet oxygen production. Photobiological assays indicate that these samples presented photodynamic antimicrobial activity against both gram-positive and gram-negative bacterial and some Candida ssp. yeast strains at sub-inhibitory concentrations. The susceptibility of gram-negative bacteria was significantly enhanced when CaCl2 or MgCl2 were employed. Greater energy doses and double sample's dosage also decreased microbial survival. It is suggested that GB1 photodynamic activity happens through both types I and II photochemical mechanisms, but with a predominance of the latter. Phytoderivatives of G. blepharophylla promoted antimicrobial effect, however more detailed study concerning chemical composition of the crude extracts and fractions as also photophysical and photochemical characteristics of GB1 are necessary to ensure their potential as photosensitizers at antimicrobial photodynamic inactivation. (C) 2016 Elsevier B.V. All rights reserved. 160 154 162 Fundo de Apoio ao Ensino a Pesquisa e Extensao-UNICAMP Fundacao de Amparo a Pesquisa do Estado de Sao Paulo Conselho Nacional de Desenvolvimento Cientifico e Tecnologico Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)