Artículos de revistas
Selenium Bioaccessibility And Speciation In Selenium-enriched Lettuce: Investigation Of The Selenocompounds Liberated After In Vitro Simulated Human Digestion Using Two-dimensional Hplc-icp-ms
Registro en:
Journal Of Agricultural And Food Chemistry. Amer Chemical Soc, v. 65, p. 3031 - 3038, 2017.
0021-8561
1520-5118
WOS:000399354000014
10.1021/acs.jafc.7b01188
Autor
da Silva
Emanueli do Nascimento; Aureli
Federica; D'Amato
Marilena; Raggi
Andrea; Cadore
Solange; Cubadda
Francesco
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The evaluation of selenium-enriched vegetables as potential dietary sources of selenium, an essential element for humans, requires an assessment of the plant's accumulation ability as well as of the bioaccessibility and speciation of the accumulated selenium, which influence its biological effects in humans. Lettuce hydroponically grown at three selenite (SeVI)/selenate (SeIV) amendment levels was characterized accordingly. Selenium accumulation in lettuce leaves was greatest with Se(VI) amendment, whereas bioaccessibility was 70% on average in both cases. Selenium speciation in gastrointestinal hydrolysates, characterized by anion and cation exchange HPLC-ICP-MS, showed that Se(IV) was largely biotransformed into organoselenium metabolites, with selenomethionine accounting for 1/3 of the total detected species, whereas Se(VI) was incorporated as such in the edible portion of the plant, with only a small fraction (similar to,20%) converted into organic species. Taking into account both nutritional quality and safety, the Se(IV)-enriched lettuce appeared more favorable as a potential selenium source for human consumption. 65 14 3031 3038 Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/26855-2, 2012/15020-4, 2014/15425-0] Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)