Artículos de revistas
Cytotoxic Effect Of Erythroxylum Daphnites Extract Is Associated With G(1) Cell Cycle Arrest And Apoptosis In Oral Squamous Cell Carcinoma
Registro en:
1551-4005
Cell Cycle. TAYLOR & FRANCIS INC, n. 15, n. 7, p. 948 - 956.
1538-4101
WOS:000374997000013
10.1080/15384101.2016.1151583
Autor
Elias
ST; Macedo
CCS; Simeoni
LA; Silveira
D; Magalhaes
PO; Lofrano-Porto
A; Coletta
RD; Neves
FAR; Guerra
ENS
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Plant-derived molecules showing antineoplastic effects have recently gained increased attention as potential adjuvants to traditional therapies for various cancers. Cerrado biome in Brazil contains high floral biodiversity, but knowledge about the potential therapeutic effects of compounds derived from that flora is still limited. The present study investigated the antineoplastic activity of Erythroxylum daphnites Mart., a Brazilian native plant from Cerrado biome, in the SCC-9 oral squamous cell carcinoma cell line. Cells were treated with various concentrations of hexane extract of Erythroxylum daphnites leaves (EDH) and assessed for cytotoxicity, proliferation, and apoptosis. Thin layer chromatography was conducted to characterize the substances present in EDH. Our results showed that EDH exerted anti-proliferative effects in SCC-9 cells by stabilizing the cell cycle at G(1) phase in association with reduced intracellular levels of cyclins D and E and increased level of p21. EDH also demonstrated pro-apoptotic properties, as shown by an increased expression of caspase-3. Triterpenes were the major constituents of EDH. Our findings demonstrated a cytotoxic effect of EDH against SCC-9 cells in vitro mediated by the restraint of cellular proliferation and induction of apoptosis. Taken together, these findings support EDH constituents as potential therapeutic adjuvants for oral cancer. 15
948 956 Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq (MCT/CNPq/FNDCT/FAPs/MEC/CAPES/PRO-CENTRO-OESTE) [564658/2010-3, 564208/2010-8] Fundacao de Amparoa a Pesquisa-Distrito Federal (FAP-DF) [193.000.484/2011] Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)