Artículos de revistas
Nanowire Arrays As Cell Force Sensors To Investigate Adhesin-enhanced Holdfast Of Single Cell Bacteria And Biofilm Stability
Registro en:
1530-6992
Nano Letters. AMER CHEMICAL SOC, n. 16, n. 7, p. 4656 - 4664.
1530-6984
WOS:000379794200099
10.1021/acs.nanolett.6b01998
Autor
Sahoo
PK; Janissen
R; Monteiro
MP; Cavalli
A; Murillo
DM; Merfa
MV; Cesar
CL; Carvalho
HF; de Souza
AA; Bakkers
EPAM; Cotta
MA
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Surface attachment of a planktonic bacteria, mediated by adhesins and extracellular polymeric substances (EPS), is a crucial step for biofilm formation. Some pathogens can modulate cell adhesiveness, impacting host colonization and virulence. A framework able to quantify cell-surface interaction forces and their dependence on chemical surface composition may unveil adhesiveness control mechanisms as new targets for intervention and disease control. Here we employed InP nanowire arrays to dissect factors involved in the early stage biofilm formation of the phytopathogen Xylella fastidiosa. Ex vivo experiments demonstrate single-cell adhesion forces up to 45 nN, depending on the cell orientation with respect to the surface. Larger adhesion forces occur at the cell poles; secreted EPS layers and filaments provide additional mechanical support. Significant adhesion force enhancements were observed for single cells anchoring a biofilm and particularly on XadA1 adhesincoated surfaces, evidencing molecular mechanisms developed by bacterial pathogens to create a stronger holdfast to specific host tissues. 16
4656 4664 FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil) [2010/51748-7, 2013/02300-1] CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil) [479486/2012-3] CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazil) FAPESP FAPESP [08/57906-3] CNPq [573913/2008-0] Dutch Technology Foundation STW of The Netherlands Organisation for Scientific Research (NWO) [11826] Dutch Ministry of Economic Affairs Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)