Effects Of Fish Oil Containing Eicosapentaenoic Acid And Docosahexaenoic Acid On Dystrophic Mdx Mice Hearts At Later Stages Of Dystrophy

dc.creatorMauricio
dc.creatorAF; Pereira
dc.creatorJA; Neto
dc.creatorHS; Marques
dc.creatorMJ
dc.date2016
dc.date2016-12-06T18:30:36Z
dc.date2016-12-06T18:30:36Z
dc.date.accessioned2018-03-29T02:03:10Z
dc.date.available2018-03-29T02:03:10Z
dc.identifier1873-1244
dc.identifierNutrition. ELSEVIER SCIENCE INC, n. 32, n. 7-8, p. 855 - 862.
dc.identifier0899-9007
dc.identifierWOS:000377058700019
dc.identifier10.1016/j.nut.2016.01.015
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0899900716000484
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/320074
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1310840
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionIn the present study, we investigated whether omega-3 would be effective against dystrophic cardiomyopathy at later stages (13 and 17 mo) of the disease. Methods: Mdx mice (8 mo old) received omega-3 oil (commercially available fish oil; FDC vitamins; omega-3) for 5 mo. Untreated-mdx mice received mineral oil. Heart and diaphragm muscle were evaluated by morphometric (fibrosis), molecular (western blot, inflammatory markers), biochemical (creatine kinase), and functional (electrocardiogram) analyses. Results: Mdx mice presented elevated plasma levels of cardiac creatine kinase (41.2 U/L in normal x 119.6 U/L in untreated-mdx mice), which were significantly decreased by omega-3 treatment. Heart fibrosis was significantly ameliorated by omega-3 treatment at 17 mo of age (untreated-mdx: 20.8% of fibrosis; omega-3-treated: 15.7% of fibrosis in right ventricle). Omega-3 improved some electrocardiogram parameters. Markers of inflammation (tumor necrosis factor alpha, matrix metalloprotease-9, and tissue inhibitor of metalloprotease 1) in mdx heart were significantly decreased by omega-3 treatment. Omega-3 increased beta-dystroglycan levels in mdx heart and did not affect the levels of the profibrotic transforming growth factor beta. Omega-3 ameliorated the dystrophic diaphragm in almost all of the parameters evaluated (fibrosis, transforming growth factor beta, metalloprotease-9, and tissue inhibitor of metalloprotease 1). Conclusions: The present study suggests that omega-3 may be useful in ameliorating dystrophic cardiomyopathy and diaphragm dystrophy in mdx mice at later stages of the disease, further supporting the use of omega-3 in DMD clinical trials. (C) 2016 Elsevier Inc. All rights reserved.
dc.description32
dc.description
dc.description855
dc.description862
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [08/58491-1, 11/51697-6, 2014/04782-6]
dc.descriptionConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [302831/2013-4, 303320/2013-3]
dc.descriptionCNPq [142282/2012-0]
dc.descriptionFAPESP [2012/13577-1]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description
dc.description
dc.description
dc.languageEnglish
dc.publisherELSEVIER SCIENCE INC
dc.publisherNEW YORK
dc.relationNutrition
dc.rightsfechado
dc.sourceWOS
dc.subjectCardiomyopathy
dc.subjectDmd
dc.subjectHeart
dc.subjectMmp-9
dc.subjectOmega-3
dc.subjectTgf-beta
dc.titleEffects Of Fish Oil Containing Eicosapentaenoic Acid And Docosahexaenoic Acid On Dystrophic Mdx Mice Hearts At Later Stages Of Dystrophy
dc.typeArtículos de revistas


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