Artículos de revistas
Baccharis Dracunculifolia-based Mouthrinse Alters The Exopolysaccharide Structure In Cariogenic Biofilms
Registro en:
1879-0003
International Journal Of Biological Macromolecules. ELSEVIER SCIENCE BV, n. 84, p. 301 - 307.
1879-0003
WOS:000369456800039
10.1016/j.ijbiomac.2015.12.015
Autor
Aires
CP; Sassaki
GL; Santana
AP; Spadaro
ACC; Cury
JA
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Baccharis dracunculifolia is a native plant from Brazil with antimicrobial activity. The purpose of this study was to investigate whether a B. dracunculifolia-based mouthrinse (Bd) changes the structure of insoluble exopolysaccharides (IEPS) in Streptococcus mutans UA159 cariogenic biofilm. Biofilms were grown on glass slides and treated with Bd, its vehicle (VC), chlorhexidine digluconate (CHX), or saline solution (NaCl). Among the treatments, only CHX significantly reduced the biofilm biomass and bacterial viability (p < 0.05). Gas chromatography mass spectrometry and nuclear magnetic resonance analyses revealed that IEPS from the four biofilm samples were alpha-glucans containing different proportions of (1 6) and (I 3) glycosidic linkages. The structural differences among the four IEPS were compared by principal component analysis (PCA). PCA analysis indicated that IEPS from VC- and NaCl-treated biofilms were structurally similar to each other. Compared with the control, IEPS from Bd- and CHX-treated biofilms were structurally different and had distinct chemical profiles. In summary, the fact that Bd changed the IEPS chemical composition indicates that this mouthrinse may affect the cariogenic properties of the S. mutans biofilm formed. (C) 2015 Published by Elsevier B.V. 84
301 307 Sao Paulo Research Foundation (FAPESP) [2010/12456-0] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)