dc.creatorSudano
dc.creatorMJ; Rascado
dc.creatorTDS; Tata
dc.creatorA; Belaz
dc.creatorKRA; Santos
dc.creatorVG; Valente
dc.creatorRS; Mesquita
dc.creatorFS; Ferreira
dc.creatorCR; Araujo
dc.creatorJP; Eberlin
dc.creatorMN; Landim-Alvarenga
dc.creatorFDC
dc.date2016
dc.date2016-12-06T18:29:41Z
dc.date2016-12-06T18:29:41Z
dc.date.accessioned2018-03-29T02:02:12Z
dc.date.available2018-03-29T02:02:12Z
dc.identifier1879-3231
dc.identifierTheriogenology. ELSEVIER SCIENCE INC, n. 86, n. 2, p. 472 - 484.
dc.identifier1879-3231
dc.identifierWOS:000377843400002
dc.identifier10.1016/j.theriogenology.2016.03.025
dc.identifierhttp://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0093691X16001448
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/319835
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1310601
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionMammalian preimplantation embryonic development is a complex, conserved, and well orchestrated process involving dynamic molecular and structural changes. Understanding membrane lipid profile fluctuation during this crucial period is fundamental to address mechanisms governing embryogenesis. Therefore, the aim of the present work was to perform a comprehensive assessment of stage-specific lipid profiles during early bovine embryonic development and associate with the mRNA abundance of lipid metabolism related genes (ACSL3, ELOVL5, and ELOVL6) and with the amount of cytoplasmic lipid droplets. Immature oocytes were recovered from slaughterhouse-derived ovaries, two-cell embryos, and eight- to 16-cell embryos, morula, and blastocysts that were in vitro produced under different environmental conditions. Lipid droplets content and mRNA transcript levels for ACSL3, ELOVL5, and ELOVL6, monitored by lipid staining and quantitative polymerase chain reaction, respectively, increased at morula followed by a decrease at blastocyst stage. Relative mRNA abundance changes of ACSL3 were closely related to cytoplasmic lipid droplet accumulation. Characteristic dynamic changes of phospholipid profiles were observed during early embryo development and related to unsaturation level, acyl chain length, and class composition. ELOVL5 and ELOVL6 mRNA levels were suggestive of over expression of membrane phospholipids containing elongated fatty acids with 16, 18, and 20 carbons. In addition, putative biomarkers of key events of embryogenesis, embryo lipid accumulation, and elongation were identified. This study provides a comprehensive description of stage-specific lipidome signatures and proposes a mechanism to explain its potential relationship with the fluctuation of both cytoplasmic lipid droplets content and mRNA levels of lipid metabolism-related genes during early bovine embryo development (c) 2016 Elsevier Inc. All rights reserved.
dc.description86
dc.description
dc.description472
dc.description484
dc.descriptionConselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq, Brasil [446647/2014-4, 162598/2013-0, 167908/2013-8]
dc.descriptionFundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP [2011/06191-7, 2012/07206-0]
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description
dc.description
dc.description
dc.languageEnglish
dc.publisherELSEVIER SCIENCE INC
dc.publisherNEW YORK
dc.relationTheriogenology
dc.rightsfechado
dc.sourceWOS
dc.subjectEmbryogenesis
dc.subjectMembrane Phospholipids
dc.subjectCytoplasmic Lipid Droplets
dc.subjectLong Chain Acyi-coa Synthetizes
dc.subjectElongation Of Very Long Fatty Acids
dc.titleLipidome Signatures In Early Bovine Embryo Development
dc.typeResenha


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