Artículos de revistas
Molecular cloning and insecticidal effect of Inga laurina trypsin inhibitor on Diatraea saccharalis and Heliothis virescens
Registro en:
Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology. Elsevier, v.156, n.41367, p.148-158, 2012
1532-0456
WOS:000309434200003
10.1016/j.cbpc.2012.07.007
Autor
Ramos, Vanessa da S.
Cabrera, Odalys G.
Camargo, Eduardo L. O.
Ambrosio, Alinne B.
Vidal, Ramon O.
da Silva, Desiree S.
Guimaraes, Lays C.
Marangoni, Sergio
Parra, Jose R. P.
Pereira, Goncalo A. G.
Macedo, Maria L. R.
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Native Inga laurina (Fabaceae) trypsin inhibitor (ILTI) was tested for anti-insect activity against Diatraea saccharalis and Heliothis virescens larvae. The addition of 0.1% ILTI to the diet of D. saccharalis did not alter larval survival but decreased larval weight by 51%. The H. virescens larvae that were fed a diet containing 0.5% ILTI showed an 84% decrease in weight. ILTI was not digested by the midgut proteinases of either species of larvae. The trypsin levels were reduced by 55.3% in the feces of D. saccharalis and increased by 24.1% in the feces of H. virescens. The trypsin activity in both species fed with ILTI was sensitive to the inhibitor, suggesting that no novel proteinase resistant to ILTI was induced. Additionally, ILTI exhibited inhibitory activity against the proteinases present in the larval midgut of different species of Lepidoptera. The organization of the ilti gene was elucidated by analyzing its corresponding genomic sequence. The recombinant ILTI protein (reILTI) was expressed and purified, and its efficacy was evaluated. Both native ILTI and reILTI exhibited a similar strong inhibitory effect on bovine trypsin activity. These results suggest that ILTI presents insecticidal properties against both insects and may thus be a useful tool in the genetic engineering of plants. (c) 2012 Elsevier Inc. All rights reserved. 156 03/abr 148 158 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FUNDECT (Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado do Mato Grosso do Sul) PROPP/UFMS (Pro-Reitoria de Pesquisa e Pos-graduacao da Universidade Federal de Mato Grosso do Sul) FINEP (Financiamento de Estudos e Projetos/Ministerio da Ciencia e Tecnologia) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)