Artículos de revistas
Structural characterization of exopolysaccharides from biofilm of a cariogenic streptococci
Registro en:
CARBOHYDRATE POLYMERS, v.84, n.4, p.1215-1220, 2011
0144-8617
10.1016/j.carbpol.2010.12.076
Autor
AIRES, Carolina P.
TENUTA, Livia M.
CARBONERO, Elaine R.
SASSAKI, Guilherme L.
IACOMINI, Marcello
CURY, Jaime A.
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Soluble (EPS-SOL), as well as insoluble extracellular polysaccharide (EPS-INSOL), extracted from biofilm of Streptococcus mutans, were analyzed by nuclear magnetic resonance spectroscopy, methylation analysis, and a controlled Smith degradation. EPS-SOL was a branched alpha-glucan containing a (1 -> 6)-and (1 -> 3)-linkages. EPS-INSOL was a branched alpha-glucan with similar linkages, but with a (1 -> 3)-linked main-chain partially substituted at O-6 with Glcp-(1 -> 6)-Glcp-side chains. Biofilm EPS had a distinct chemical structure compared with those synthesized by plankton cells or by purified enzymes from S. mutans, which could indicate different mechanisms for its degradation. (C) 2011 Published by Elsevier Ltd. 84 4 1215 1220 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)