Artículos de revistas
Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3
Registro en:
Febs Letters. Elsevier, v.586, n.19, p.3522-3528, 2012
0014-5793
WOS:000309314200080
10.1016/j.febslet.2012.08.012
Autor
Bigarella, Carolina L.
Vieira Ferro, Karla P.
Barcellos, Karin S. A.
Martins-de-Souza, Daniel
Traina, Fabiola
Novello, Jose C.
Olalla Saad, Sara T.
Archangelo, Leticia Froehlich
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. 586 19 3522 3528 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)