Artículos de revistas
Leaf Blade Area Of Different Plants Estimated By Linear And Dry Matter Measures, Calibrated With The Imagej Software
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Leaf Blade Area Of Different Plants Estimated By Linear And Dry Matter Measures, Calibrated With The Imagej Software. Interciencia, v. 40, p. 570-575 AUG-2015.
0378-1844
WOS:000359824200011
Autor
Ramos
Fabricio Tomaz; Ferreira
Lucas de Souza; Pivetta
Fernando; de Souza Maia
Joao Carlos
Institución
Resumen
Much of dry matter accumulation by plants comes from photosynthesis in the leaves. Therefore, analysis of leaf growth is useful for plant study under different climatic and soil conditions. Leaf area can be estimated with different methods and equipments, some of which are costly and time consuming, ineffective for common research conditions. However, there can be alternatives, economic and fast, through the use of imaging software, free or licensed. Thus, we aimed to estimate the area of the leaf blade of different plants based on linear and dry weight measures, calibrated with the free software ImageJ. The leaves of cotton plants (Gossypium hirsutum L) var. IMACD 6001, cashew (Anacardium occidentale L) var. FAGA 10, soybean (Glycine max L) var. M-SOY 7639RR, and corn (Zea mays L) var. Penta TL were collected from the lower third, middle and top in different phenological phases. It was found that images with a resolution of at least 7.2 megapixels can be used to estimate the actual leaf blade area, processed with the ImageJ software till 324cm(2). For larger surfaces, we recommend testing with higher resolution camera, or cut a large sheet into pieces up to 324cm(2). We observed a good agreement in the estimation of the leaf blade area based on linear and dry mass measures and, therefore, it is recommended to estimate leaf area leaves with damaged pieces or measures from several samples of leaves collected at the field based on the dry weight of the leaves, as it is operationally viable. 40 8
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