Artículos de revistas
Superoxide Anion Radical: Generation And Detection In Cellular And Non-cellular Systems
Registro en:
Superoxide Anion Radical: Generation And Detection In Cellular And Non-cellular Systems. Bentham Science Publ Ltd, v. 22, p. 4234-4256 2015.
0929-8673
WOS:000366483100001
10.2174/0929867322666151029104311
Autor
Chiste
Renan Campos; Freitas
Marisa; Mercadante
Adriana Zerlotti; Fernandes
Eduarda
Institución
Resumen
The production of superoxide anion radical (O-2 center dot(-)) is essential for the life of aerobic organisms. This free radical acts as a signaling molecule, regulating numerous biological processes including apoptosis, aging, and senescence. Nevertheless, when overproduction of O-2 center dot(-) occurs and/or antioxidant defences are deficient, oxidative stress may develop, damaging important biomolecules and altering their physiological function. These effects have been associated to the development of several diseases. Scavenging of O-2 center dot(-) has been used as a hallmark to test the antioxidant capacity of several types of compounds in cellular and non-cellular systems. However, despite the pathophysiological importance of O-2 center dot(-), the information about its endogenous and/or chemical generation and detection is dispersed and there are no reports that concisely cover the information in an integrated form. This gap can explain the limitations attributed to the currently used systems, namely in what concerns the selectivity, specificity and validation. This review attempts to provide a critical assessment of the available O-2 center dot(-) generating and detection, both in endogenous and chemical systems, scrutinizing its advantages and limitations in order to facilitate the choice and implementation of the O-2 center dot(-) generator and/or detection method that better fits the researchers' objectives. 22 37
4234 4256 FCT (Fundacao para a Ciencia e Tecnologia, Portugal) [UID/Multi/04378/2013, PTDC/QEQ-QAN/1742/2014] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FSE FCT [SFRH/BPD/76909/2011] national funds of MCTES