Artículos de revistas
Cellular Senescence And Autophagy Of Myoepithelial Cells Are Involved In The Progression Of In Situ Areas Of Carcinoma Ex-pleomorphic Adenoma To Invasive Carcinoma. An In Vitro Model
Registro en:
Cellular Senescence And Autophagy Of Myoepithelial Cells Are Involved In The Progression Of In Situ Areas Of Carcinoma Ex-pleomorphic Adenoma To Invasive Carcinoma. An In Vitro Model. Springer, v. 9, p. 255-265 SEP-2015.
1873-9601
WOS:000363377200006
10.1007/s12079-015-0291-9
Autor
Barcellos Silva
Carolina Amalia; Martinez
Elizabeth Ferreira; Dias Demasi
Ana Paula; Altemani
Albina; da Silveira Bossonaro
Jeruza Pinheiro; Araujo
Ney Soares; de Araujo
Vera Cavalcanti
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) During tumor invasion, benign myoepithelial cells of carcinoma ex-pleomorphic adenoma (CXPA) surround malignant epithelial cells and disappear. The mechanisms involved in the death and disappearance of these myoepithelial cells were investigated via analysis of the expression of regulatory proteins for apoptosis, autophagy and cellular senescence in an in situ in vitro model. Protein expression relating to apoptosis (Bax, Bcl-2, Survivin), autophagy (Beclin-1, LC3B) and cellular senescence (p21, p16) was evaluated using indirect immunofluorescence. beta-galactosidase expression was assessed via histochemistry. Biopsies of CXPA (ex vivo) allowed immunhistochemical evaluation of p21 and p16, whilst LC3B, p21 and p16 protein expression was analyzed by western blotting. In the in vitro model, the myoepithelial cells were positive for LC3B (cytoplasm) and p21 (nucleus), whilst in vivo positivity for p21 and p16 was observed. In vitro, beta-galactosidase activity increased in the myoepithelial cells over time. Western blotting analysis revealed an increased LC3B, p16 and p21 expression in the myoepithelial cells with previous contact with the malignant cells when compared with those without contact. The investigation of behavior of benign myoepithelial cells in ductal areas of CXAP revealed that the myoepithelial cells are involved in the autophagy-senescence phenotype that subsequently leads to their disappearance. 9 3
255 265 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FAPESP [2011/21157-0] CNPq [473939/2011-8]