Artículos de revistas
Downhill Running-based Overtraining Protocol Improves Hepatic Insulin Signaling Pathway Without Concomitant Decrease Of Inflammatory Proteins
Registro en:
Downhill Running-based Overtraining Protocol Improves Hepatic Insulin Signaling Pathway Without Concomitant Decrease Of Inflammatory Proteins. Public Library Science, v. 10, p. OCT-2015.
1932-6203
WOS:000362510600126
10.1371/journal.pone.0140020
Autor
da Rocha
Alisson L.; Pereira
Bruno C.; Pauli
Jose R.; Cintra
Dennys E.; de Souza
Claudio T.; Ropelle
Eduardo R.; da Silva
Adelino S. R.
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The purpose of this study was to verify the effects of overtraining (OT) on insulin, inflammatory and gluconeogenesis signaling pathways in the livers of mice. Rodents were divided into control (CT), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up) and overtrained by running without inclination (OTR). Rotarod, incremental load, exhaustive and grip force tests were used to evaluate performance. Thirty-six hours after a grip force test, the livers were extracted for subsequent protein analyses. The phosphorylation of insulin receptor beta (pIRbeta), glycogen synthase kinase 3 beta (pGSK3beta) and forkhead box O1 (pFoxo1) increased in OTR/down versus CT. pGSK3beta was higher in OTR/up versus CT, and pFoxo1 was higher in OTR/up and OTR versus CT. Phosphorylation of protein kinase B (pAkt) and insulin receptor substrate 1 (pIRS-1) were higher in OTR/up versus CT and OTR/down. The phosphorylation of I kappa B kinase alpha and beta (pIK-Kalpha/beta) was higher in all OT protocols versus CT, and the phosphorylation of stress-activated protein kinases/Jun amino-terminal kinases (pSAPK-JNK) was higher in OTR/ down versus CT. Protein levels of peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC-1alpha) and hepatocyte nuclear factor 4alpha (HNF-4alpha) were higher in OTR versus CT. In summary, OTR/down improved the major proteins of insulin signaling pathway but up-regulated TRB3, an Akt inhibitor, and its association with Akt. 10 10
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP [2013/20591-3, 2014/25459-9, 2013/22737-5, 2013/19985-7]