Artículos de revistas
Green Propolis Modulates Gut Microbiota, Reduces Endotoxemia And Expression Of Tlr4 Pathway In Mice Fed A High-fat Diet
Registro en:
Green Propolis Modulates Gut Microbiota, Reduces Endotoxemia And Expression Of Tlr4 Pathway In Mice Fed A High-fat Diet. Elsevier Science Bv, v. 76, p. 796-803 OCT-2015.
0963-9969
WOS:000361924500057
10.1016/j.foodres.2015.07.026
Autor
Roquetto
Aline Rissetti; Santana Monteiro
Naice Eleidiane; Moura
Carolina Soares; Toreti
Viviane Cristina; de Pace
Fernanda; dos Santos
Andrey; Park
Yong Kun; Amaya-Farfan
Jaime
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Due to the various beneficial effects attributed to propolis, which include anti-inflammatory and anti-bacterial infection properties, the objective of the study was to evaluate the effect of propolis supplementation on the composition of the intestinal microbiota and its anti-inflammatory action. Forty male C57BL/6 mice were fed either a standard diet (control), a high-fat (HF) diet, or a high-fat diet supplemented with 0.2% crude propolis (HFP) for 2 or 5 weeks prior to sacrifice. Blood samples were collected for the determination of lipopolysaccharide (LPS) and classical biochemical parameters. Expression of the TLR4 pathway in muscle, and DNA sequencing for the 16S rRNA of the gut microbiota were performed. The HF diet increased the proportion of the phylum Firmicutes and inflammatory biomarkers, while supplementation with propolis for five weeks rendered the microbiota profile nearly normal. Consistently with the above, the supplementation reduced levels of circulating LPS and down-regulated the TLR4 pathway and inflammatory cytokine expressions in muscle. Moreover, propolis improved such biochemical parameters as serum triacylglycerols and glucose levels. The data suggest that propolis supplementation reduces inflammatory response and endotoxemia by preventing dysbiosis in mice challenged with a high-fat diet. (C) 2015 Elsevier Ltd. All rights reserved. 76
796 803 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) FAPESP [2012/05859-7]