Artículos de revistas
Nanofilms Of Hyaluronan/chitosan Assembled Layer-by-layer: An Antibacterial Surface For Xylella Fastidiosa
Registro en:
Nanofilms Of Hyaluronan/chitosan Assembled Layer-by-layer: An Antibacterial Surface For Xylella Fastidiosa. Elsevier Sci Ltd, v. 136, p. 1-11 Jan-2016.
0144-8617
WOS:000365972000001
10.1016/j.carbpol.2015.08.076
Autor
Hernandez-Montelongo
Jacobo; Nascimento
Vicente F.; Murillo
Duber; Taketa
Thiago B.; Sahoo
Prasana; de Souza
Alessandra A.; Beppu
Marisa M.; Cotta
Monica A.
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) In this work, nanofilms of hyaluronan/chitosan (HA/CHI) assembled layer by layer were synthesized; their application as a potential antimicrobial material was demonstrated for the phytopathogen Xylella fastidiosa, a gram-negative bacterium, here used as a model. For the synthesis, the influence of pH and ionic strength of these natural polymer stem-solutions on final characteristics of the HA/CHI nanofilms was studied in detail. The antibacterial effect was evaluated using widefield fluorescence microscopy. These results were correlated with the chemical properties of the nanofilms, studied by FTIR and Raman spectroscopy, as well as with their morphology and surface properties characterized using SEM and AFM. The present findings can be extended to design and optimize HA/CHI nanofilms with enhanced antimicrobial behavior for other type of phytopathogenic gram-negative bacteria species, such as Xanthomonas citri, Xanthomas campestri and Ralstonia solanacearum. (C) 2015 Elsevier Ltd. All rights reserved. 136
1 11 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) FAPEAM Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) FAPESP [2010/51748-7, 2013/02300-1]