Artículos de revistas
Elimination Of The Artefact Peaks In Capillary Electrophoresis Determination Of Glutamate By Using Organic Solvents In Sample Preparation
Registro en:
Elimination Of The Artefact Peaks In Capillary Electrophoresis Determination Of Glutamate By Using Organic Solvents In Sample Preparation. Wiley-v C H Verlag Gmbh, v. 38, p. 3781-3787 NOV-2015.
1615-9306
WOS:000363480800015
10.1002/jssc.201500601
Autor
Madeira Campos
Camila Dalben; de Campos Braga
Patricia Aparecida; Reyes Reyes
Felix Guillermo; Fracassi da Silva
Jose Alberto
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Focusing on the demand from the food industry for fast and reliable alternative methods to control the quality of food products, we present in this paper a method for amino acid separation and glutamic acid quantification in complex matrices employing capillary electrophoresis with capacitively coupled contactless conductivity detection. We demonstrate by simulation and experimentally the use of organic solvents in sample preparation to prevent peak splitting and increase stacking in capillary electrophoretic separations of amino acids. Additionally, we obtained results for glutamic acid quantification comparable to those obtained via traditional methods used at industrial sites. We tested premium and low-cost samples with large variations in their glutamic acid content, which demonstrated the wide range of applicability of the method presented herein. The results of the proposed capacitively coupled contactless conductivity detection based capillary electrophoresis method agreed with those obtained by an enzymatic detector and ultra high performance liquid chromatography coupled to tandem mass spectrometry, considering a confidence level of 95%. 38 21
3781 3787 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Instituto Nacional de Ciencia e Tecnologia em Bioanalitica (INCTBio) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FAPESP [2011/02477-3] CNPq [309449/2014-6]