Artículos de revistas
Comparative Study Of The Bioconversion Process Using R-(+)- And S-(-)-limonene As Substrates For Fusarium Oxysporum 152b
Registro en:
Comparative Study Of The Bioconversion Process Using R-(+)- And S-(-)-limonene As Substrates For Fusarium Oxysporum 152b. Elsevier Sci Ltd, v. 174, p. 606-613 MAY-2015.
0308-8146
WOS:000348088000083
10.1016/j.foodchem.2014.11.059
Autor
Molina
Gustavo; Bution
Murillo L.; Bicas
Juliano L.; Dolder
Mary Anne Heidi; Pastore
Glaucia M.
Institución
Resumen
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study compared the bioconversion process of S-(-)-limonene into limonene-1,2-diol with the already established biotransformation of R-(+)-limonene into alpha-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B. The bioconversion of the S-(-)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. When submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limonene-1,2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment, indicating a complete loss of cell viability. Furthermore, the results showed that this process can be characterised as an aerobic system that was catalysed by limonene-1,2-epoxide hydrolase, had an intracellular nature and was cofactor-dependent because the final product was not detected by an anaerobic process. Finally, this is the first report to characterise the bioconversion of R-(+)- and S-(-)-limonene by cellular detoxification using ultra-structural analysis. (C) 2014 Elsevier Ltd. All rights reserved. 174
606 613 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FAPESP [2011/50687-7] CNPq [481487/2011-5, 473981/2012-2]