Artículos de revistas
Revealing Praziquantel Molecular Targets Using Mass Spectrometry Imaging: An Expeditious Approach Applied To Schistosoma Mansoni
Registro en:
Revealing Praziquantel Molecular Targets Using Mass Spectrometry Imaging: An Expeditious Approach Applied To Schistosoma Mansoni. Elsevier Sci Ltd, v. 45, p. 385-391 MAY-2015.
0020-7519
WOS:000353743400005
10.1016/j.ijpara.2014.12.008
Autor
Ferreira
Monica Siqueira; de Oliveira
Rosimeire Nunes; de Oliveira
Diogo Noin; Esteves
Cibele Zanardi; Allegretti
Silmara Marques; Catharino
Rodrigo Ramos
Institución
Resumen
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Finding specific molecular targets and the mechanism of action of praziquantel in the treatment of schistosomiasis remains a challenging task. Our efforts were focused on obtaining further information on worm composition before and after exposure to praziquantel in the treatment of schistosomiasis to elucidate the potential sites of action of this drug. Evidence indicates that the lipid bilayer is changed by treatment with praziquantel. Following this rationale, we employed a mass spectrometry imaging-based approach that helped to characterise lipids in specific locations, which are directly involved in the biochemical pathways of the BH strain of Schistosoma mansoni, as well as differentiating the molecular response that each worm sex presents in vivo. Our findings demonstrated significant differences between the chemical markers found in adult worms before and after praziquantel exposure, especially in phospholipids, which were predominantly identified as chemical markers in all samples. Results also indicate that distinct molecular pathways in both male and female worms could be differentially affected by praziquantel treatment. These data shine new light on the mechanism of action of praziquantel, taking a further step towards its full understanding. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. 45 6
385 391 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) National Science and Technology Institutes (INCT), Brazil Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP [11/50400-0, 14/00302-0]