Artículos de revistas
Epitopes Resistance To The Simulated Gastrointestinal Digestion Of Beta-lactoglobulin Submitted To Two-step Enzymatic Modification
Registro en:
Epitopes Resistance To The Simulated Gastrointestinal Digestion Of Beta-lactoglobulin Submitted To Two-step Enzymatic Modification. Elsevier Science Bv, v. 72, p. 191-197 JUN-2015.
0963-9969
WOS:000356122600024
10.1016/j.foodres.2015.03.044
Autor
Villas-Boas
Mariana Battaglin; Benede
Sara; Zollner
Ricardo de Lima; Netto
Flavia Maria; Molina
Elena
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The effects beta-lactoglobulin (beta Lg) hydrolysis with alcalase or bromelain and its association with polymerization by transglutaminase (TG) on both the protein antigenicity and resistance of epitopes to in vitro digestion were investigated. Despite the polymerization with TG altered the gastric and gastrointestinal (GI) digestion patterns of the hydrolysates with bromelain, no changes were observed with alcalase. Before and after in vitro digestion, native beta Lg showed the highest IgE-binding capacity, evaluated using sera from milk-allergic patients, while the enzymatic treatments-associated or not-reduced the beta Lg antigenicity response. Seven and four epitopes were found after gastric and GI digestion of native beta Lg, respectively. After gastric digestion of the hydrolysates with bromelain, two epitopes were identified, being Tyr(42)-Leu(54) resistant to GI digestion. No epitope was found in GI digested products of hydrolysates with alcalase. Hydrolysis associated or not with polymerization reduced the number of epitopes and the IgE-binding capacity of the samples. (C) 2015 Elsevier Ltd. All rights reserved. 72
191 197 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) CSIC through JAE Pre Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) CNPq [AGL2011-24740]