Leishmaniasis is a vector-borne zoonotic disease caused by protozoa in the genus Leishmania, typical of rural and peri‑urban environments. The causative agent of American visceral leishmaniasis is Leishmania (Leishmania) infantum chagasi and the main insect vector in Brazil is Lutzomyia longipalpis. Dogs (Canis familiaris) are important in the transmission of the disease, as a reservoir closely related to humans and an infection source for phlebotomine vectors. Since 1990, an increasing number of feline leishmaniasis cases have been reported, suggesting that domestic cats (Felis catus) might be involved in the epidemiology of the disease. The present study analyzed the prevalence of anti-Leishmania spp. antibodies in naturally infected domestic cats from various neighborhoods in the municipality of Belém, Pará, Brazil, using the indirect immunofluorescence assay (IFA) and the direct agglutination test (DAT). Among the 443 samples tested, 18 (4.06%) presented positive reactions in the IFA. The observed titers were 40 IU in 4.97% of the samples and 80 IU in 0.90%. In the DAT test, positive results were found in 25 (5.64%) of the samples. The observed titers were also 40 IU (4.97%) and 80 IU (0.68%). The agreement rate between the two tests was considered low (Kappa coefficient = 0.10). © 2015, Brazilain Coll Veterinary Parasitology. All rights reserved.244464470Alves, W.A., Bevilacqua, P.D., Quality of diagnosis of canine visceral Leishmaniasis in epidemiological surveys: An epidemic in Belo Horizonte, Minas Gerais, Brazil, 1993-1997 (2004) Cad Saude Publica, 20 (1), pp. 259-265. , http://dx.doi.org/10.1590/S0102-311X2004000100043.PMid:15029328Andrade, A.R.O.D., Nunes, V.L.B., Galati, E.A.B., Arruda, C.C.P.D., Santos, M.F.D.C., Rocca, M.E.G., Epidemiological study on leishmaniasis in an area of environmental tourism and ecotourism, State of Mato Grosso do Sul, 2006-2007 (2009) Rev Soc Bras Med Trop, 42 (5), pp. 488-493. , http://dx.doi.org/10.1590/S0037-86822009000500003.PMid:19967228Baneth, G., Leishmaniases (2006) Infectious diseases of the dog and cat, pp. 685-698. , Greene CE., 5th ed. Missouri: Saunders ElsevierBrasil, Ministério da Saúde, Secretaria de Vigilância em Saúde (2007) Manual de vigilância da leishmaniose tegumentar americana [online]. Brasília: Editora do Ministério da Saúde, , http://bvsms.saude.gov.br/bvs/publicacoes/manual_vigilancia_leishmaniose_tegumentar_americana.pdf, [cited 2014 Feb 10]Brasil, Ministério da Saúde (2011) Nota técnica nº 48/2011-CGDT/DEVIT/SVS/ MS: Esclarecimentos sobre o diagnóstico sorológico da leishmaniose visceral canina utilizado na rede pública de saúde [online]. Brasília, , http://www.saude.ms.gov.br/controle/ShowFile.php?id=113354, [cited 2014 Feb 10]Bresciani, K.D.S., Serrano, A.C.M., Matos, L.V.S., Savani, E.S.M.M., D'Auria, S.R.N., Perri, S.H.V., Ocorrência de Leishmania spp. em felinos do município de Araçatuba, SP (2010) Rev Bras Parasitol Vet, 19 (2), pp. 127-129. , http://dx.doi.org/10.4322/rbpv.01902012.PMid:20624352Camargo, M.E., Introdução às técnicas de imunofluorescência (1974) Rev Bras Patol Clin, 10 (1), pp. 143-169Cardoso, L., Lopes, A.P., Sherry, K., Schallig, H., Solano-Gallego, L., Low seroprevalence of Leishmania infantum infection in cats from northern Portugal based on DAT and ELISA (2010) Vet Parasitol, 174 (1-2), pp. 37-42. , http://dx.doi.org/10.1016/j.vetpar.2010.08.022.PMid:20851524Coelho, W.M.D., Lima, V.M., Amarante, A.F., Langoni, H., Pereira, V.B., Abdelnour, A., Occurrence of Leishmania (Leishmania) chagasi in a domestic cat (Felis catus) in Andradina, São Paulo, Brazil: Case report (2010) Rev Bras Parasitol Vet, 19 (4), pp. 256-258. , http://dx.doi.org/10.1590/S1984-29612010000400013.PMid:21184705Costa, J.M.L., Epidemiologia das leishmanioses no Brasil (2005) Gaz Méd Bahia, 75 (1), pp. 3-17Costa, T.A.C., Rossi, C.N., Laurenti, M.D., Gomes, A.A.D., Vides, J.P., Vicente Sobrinho, L.S., Occurrence of leishmaniasis in cats from endemic area for visceral leishmaniasis (2010) Braz J Vet Res Anim Sci, 47 (3), pp. 213-217Courtenay, O., Quinnell, R.J., Garcez, L.M., Shaw, J.J., Dye, C., Infectiousness in a cohort of brazilian dogs: Why culling fails to control visceral leishmaniasis in areas of high transmission (2002) J Infect Dis, 186 (9), pp. 1314-1320. , http://dx.doi.org/10.1086/344312.PMid:12402201Curi, N.H.A., Miranda, I., Talamoni, S.A., Serologic evidence of Leishmania infection in free-ranging wild and domestic canids around a Brazilian National Park (2006) Mem Inst Oswaldo Cruz, 101 (1), pp. 99-101. , http://dx.doi.org/10.1590/S0074-02762006000100019.PMid:16699717Deane, L., Deane, G., Estudos sobre a leishmaniose visceral americana (1937) O Hospital, 12 (2), pp. 189-199Deane, L.M., Deane, M.P., Visceral leishmaniasis in Brazil: Geographical distribution and transmission (1962) Rev Inst Med Trop Sao Paulo, 4 (1), pp. 198-212. , PMid:13884626Duarte, A., Castro, I., Fonseca, I.M.P., Almeida, V., Carvalho, L.M., Meireles, J., Survey of infectious and parasitic diseases in stray cats at the Lisbon Metropolitan Area, Portugal (2010) J Feline Med Surg, 12 (6), pp. 441-446. , http://dx.doi.org/10.1016/j.jfms.2009.11.003.PMid:20466573Feitosa, M.A.C., Julião, G.R., Costa, M.D.P., Belém, B., Pessoa, F.A.C., Diversity of sand flies in domiciliary environment of Santarém, state of Pará, Brazil: Species composition and abundance patterns in rural and urban areas (2012) Acta Amazon, 42 (4), pp. 507-514. , http://dx.doi.org/10.1590/S0044-59672012000400008Ferreira, E.D.C., Lana, M., Carneiro, M., Reis, A.B., Paes, D.V., Silva, E.S.D., Comparison of serological assays for the diagnosis of canine visceral leishmaniasis in animals presenting different clinical manifestations (2007) Vet Parasitol, 146 (3-4), pp. 235-241. , http://dx.doi.org/10.1016/j.vetpar.2007.02.015.PMid:17403582Ferreira, J.V.S., Santos, T.V., Santos, E.M., Gorayeb, I.S., Phlebotomine sand flies (Diptera: Psychodidae) in forest fragments of Belém metropolitan area, Pará State, Brazil, with considerations on vectors of American cutaneous leishmaniasis agents (2014) Rev Pan-Amaz Saude, 5 (2), pp. 29-35. , http://dx.doi.org/10.5123/S2176-62232014000200004Ferrer, L., Aisa, M.J., Roura, X., Portús, M., Serological diagnosis and treatment of canine leishmaniasis (1995) Vet Rec, 136 (20), pp. 514-516. , http://dx.doi.org/10.1136/vr.136.20.514.PMid:7660549Figueiredo, F.B., Bonna, I.C., Nascimento, L.D., Costa, T., Baptista, C., Pacheco, T.M., Serological evaluation for detection of anti-Leishmania antibodies in dogs and cats in the district of Santa Rita de Cássia, municipality of Barra Mansa, State of Rio de Janeiro (2009) Rev Soc Bras Med Trop, 42 (2), pp. 141-145. , PMid:19448930Garcez, L.M., Cardoso, J.F., Chagas, A.P., Miranda, J.F.C., Souza, G.C.R., Soares, D.C., Vigilância da leishmaniose visceral em localidades epidemiologicamente distintas em Juruti, um município minerário do Estado do Pará, Brasil (2010) Rev Pan-Amaz Saúde, 1 (1), pp. 107-116Garcez, L.M., Silveira, F.T., El Harith, A., Lainson, R., Shaw, J.J., Experimental cutaneous leishmaniasis: IV. The humoral response of Cebus apella (Primates: Cebidae) to infections of Leishmania (Leishmania) amazonensis, L. (Viannia) lainsoni and L. (V.) braziliensis using the direct agglutination test (1997) Acta Trop, 68 (1), pp. 65-76. , http://dx.doi.org/10.1016/S0001-706X(97)00078-8.PMid:9352003Garrido, J.M.C.B.G., (2012) Contribuição para o estudo da prevalência da infecção por Leishmania infantum em gatos domésticos e errantes nos distritos de Lisboa e Viseu [Dissertation], , Lisboa: Faculdade de Medicina Veterinária, Universidade Técnica de LisboaGomes, A.P.S., Cordeiro, R.L.R., Reação cruzada no diagnóstico sorológico de leishmaniose canina (2004) Rev Bras Parasitol Vet, 23 (S1), p. 238Gramiccia, M., Gradoni, L., The current status of zoonotic leishmaniases and approaches to disease control (2005) Int J Parasitol, 35 (11-12), pp. 1169-1180. , http://dx.doi.org/10.1016/j.ijpara.2005.07.001.PMid:16162348Gramiccia, M., Recent advances in leishmaniosis in pet animals: Epidemiology, diagnostics and anti-vectorial prophylaxis (2011) Vet Parasitol, 181 (1), pp. 23-30. , http://dx.doi.org/10.1016/j.vetpar.2011.04.019.PMid:21570192Kirkpatrick, C.E., Farrell, J.P., Goldschmidt, M.H., Leishmania chagasi and L. donovani: Experimental infections in domestic cats (1984) Exp Parasitol, 58 (2), pp. 125-131. , http://dx.doi.org/10.1016/0014-4894(84)90027-4.PMid:6479284Lonardoni, M.V.C., Bernal, F.H.Z., Silveira, T.G.V., Antunes, V., Teodoro, U., Jorge, F.A., Comparação entre imunofluorescência indireta e aglutinação direta para o diagnóstico sorológico da leishmaniose tegumentar americana em cães errantes (2006) Arq Bras Med Vet Zootec, 58 (6), pp. 1001-1008. , http://dx.doi.org/10.1590/S0102-09352006000600005Luciano, R.M., Lucheis, S.B., Troncarelli, M.Z., Luciano, D.M., Langoni, H., Avaliação da reatividade cruzada entre antígenos de Leishmania spp e Trypanosoma cruzi na resposta sorológica de cães pela técnica de imunofluorescência indireta (RIFI) (2009) Braz J Vet Res Anim Sci, 46 (3), pp. 181-187Maia, C., Campino, L., Can domestic cats be considered reservoir hosts of zoonotic leishmaniasis? (2011) Trends Parasitol, 27 (8), pp. 341-344. , http://dx.doi.org/10.1016/j.pt.2011.03.008.PMid:21570915Maia, C., Gomes, J., Cristóvão, J., Nunes, M., Martins, A., Rebêlo, E., Feline Leishmania infection in a canine leishmaniasis endemic region, Portugal (2010) Vet Parasitol, 174 (3-4), pp. 336-340. , http://dx.doi.org/10.1016/j.vetpar.2010.08.030.PMid:20869810Mancianti, F., Pedonese, F., Poli, A., Evaluation of dot enzyme-linked immunosorbent assay (dot-ELISA) for the serodiagnosis of canine leishmaniosis as compared with indirect immunofluorescence assay (1996) Vet Parasitol, 65 (1-2), pp. 1-9. , http://dx.doi.org/10.1016/0304-4017(96)00946-6.PMid:8916395Mancianti, F., Feline leishmaniasis: What's the epidemiological role of the cat? (2004) Parassitologia, 46 (1-2), pp. 203-206. , PMid:15305717Marodin, N.B., (2011) Estudo da avaliação laboratorial e ocorrência da infecção pela Leishmania spp, , nos felinos domésticos de uma região periurbana Distrito Federal [Dissertation]. Brasília: Faculdade de Agronomia e Medicina Veterinária, Universidade de BrasíliaMartín-Sánchez, J.C., Acedo, M., Muñoz-Pérez, M., Pesson, B., Marchal, O., Morillas-Márquez, F., Infection by Leishmania infantum in cats: Epidemiological study in Spain (2007) Vet Parasitol, 145 (3-4), pp. 267-273. , http://dx.doi.org/10.1016/j.vetpar.2006.11.005.PMid:17157440Mikaeili, F., Fakhar, M., Sarkari, B., Motazedian, M.H., Hatam, G., Comparison of serological methods (ELISA, DAT and IFA) for diagnosis of visceral leishmaniasis utilizing an endemic strain (2007) Iran J Immunol, 4 (2), pp. 116-121. , PMid:17652852Mohebali, M., Edrissian, G.H., Shirzadi, M.R., Akhoundi, B., Hajjaran, H., Zarei, Z., An observational study on the current distribution of visceral leishmaniasis in different geographical zones of Iran and implication to health policy (2011) Travel Med Infect Dis, 9 (2), pp. 67-74. , http://dx.doi.org/10.1016/j.tmaid.2011.02.003.PMid:21419708Noé, P., (2008) Research of Leishmania sp, , infection in cats (Felis domesticus) from Campo Grande, MS, Brazil [Dissertation]. Campo Grande: Faculdade de Medicina Veterinária, Zootecnia Universidade Federal de Mato Grosso do SulPoli, A., Abramo, F., Barsotti, P., Leva, S., Gramiccia, M., Ludovisi, A., Feline leishmaniosis due to Leishmania infantum in Italy (2002) Vet Parasitol, 106 (3), pp. 181-191. , http://dx.doi.org/10.1016/S0304-4017(02)00081-X.PMid:12062507Quinnell, R.J., Courtenay, O., Garcez, L.M., Kaye, P.M., Shaw, M.A., Dye, C., IgG subclass responses in a longitudinal study of canine visceral leishmaniasis (2003) Vet Immunol Immunopathol, 91 (3-4), pp. 161-168. , http://dx.doi.org/10.1016/S0165-2427(02)00311-2.PMid:12586479Rosa, N.J.G.C., (2009) Rastreio de Dirofilariose e de Leishmaniose em gatos da área metropolitana de Lisboa [Dissertation], , Lisboa: Faculdade de Medicina Veterinária, Universidade Técnica de LisboaRossi, C.N., (2007) Ocorrência de Leishmania sp, , em gatos do município de Araçatuba-São Paulo-Brasil [Dissertation]. Jaboticabal: Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual PaulistaSAS Institute-SAS, (2011) SAS/STAT user's guide, , Version 9.3. Cary: SAS Institute IncSavani, E.S.M.M., Camargo, M.C.G.O., Carvalho, M.R., Zampieri, R.A., Santos, M.G., D'Auria, S.R.N., The first record in the Americas of an autochthonous case of Leishmania (Leishmania) infantum chagasi in a domestic cat (Felix catus) from Cotia County, Sao Paulo State, Brazil (2004) Vet Parasitol, 120 (3), pp. 229-233. , http://dx.doi.org/10.1016/j.vetpar.2004.01.008.PMid:15041097Schallig, H.D.F.H., Schoone, G.J., Beijer, E.G.M., Kroon, C.C.M., Hommers, M., Özbel, Y., Development of a fast agglutination screening test (FAST) for the detection of anti-Leishmania antibodies in dogs (2002) Vet Parasitol, 109 (1-2), pp. 1-8. , http://dx.doi.org/10.1016/S0304-4017(02)00268-6.PMid:12383620Schwanke, K., Silva, A.M.M., Pacheco, A., Bahia, M., Silveira, F.T., Scofield, A., Molecular diagnosis and frequency of anti-Leishmania infantum chagasi antibodies in dogs in Belém/Pará, Brazil (2014) Pesqui Vet Bras, 34 (3), pp. 255-260. , http://dx.doi.org/10.1590/S0100-736X2014000300010Sergent, E., Lombard, J., Quilichini, M., La leishmaniose à Alger: Infection simultanée d'un enfant, d'un chien et d'un chat dans la même habitation (1912) Bull Soc Pathol Exot, 5, pp. 93-98Silva, A.V., Cândido, C.D.S., Pereira, D.P., Brazil, R.P., Carreira, J.C., The first record of american visceral leishmaniasis in domestic cats from Rio de Janeiro, Brazil (2008) Acta Trop, 105 (1), pp. 92-94. , http://dx.doi.org/10.1016/j.actatropica.2007.09.001.PMid:17953938Silveira No, O.J., (2010) Otimização de ensaios de PCR para a detecção específica de Leishmania chagasi [Dissertation], , Goiás: Universidade Federal de GoiâniaSimões-Mattos, L., Mattos, M.R.F., Teixeira, M.J., Oliveira-Lima, J.W., Bevilaqua, C.M.L., Prata-Júnior, R.C., The susceptibility of domestic cats (Felis catus) to experimental infection with Leishmania braziliensis (2005) Vet Parasitol, 127 (3-4), pp. 199-208. , http://dx.doi.org/10.1016/j.vetpar.2004.10.008.PMid:15710520Thrusfield, M., (2007) Veterinary epidemiology, , Oxford: Blackwell ScienceUchôa, C.M.A., Serra, C.M.B., Duarte, R., Magalhães, C.M., Silva, R.M., Theophilo, F., Aspectos sorológicos e epidemiológicos da leishmaniose tegumentar americana canina em Maricá, Rio de Janeiro, Brasil (2001) Rev Soc Bras Med Trop, 34 (6), pp. 563-568. , http://dx.doi.org/10.1590/S0037-86822001000600011.PMid:11813064Valadas, S., Minervino, A.H., Lima, V.M., Soares, R.M., Ortolani, E.L., Gennari, S.M., Occurrence of antibodies anti-Neospora caninum, anti-Toxoplasma gondii, and anti-Leishmania chagasi in serum of dogs from Pará State, Amazon, Brazil (2010) Parasitol Res, 107 (2), pp. 453-457. , http://dx.doi.org/10.1007/s00436-010-1890-2.PMid:20445991Vexenat, A.D.C., Santana, J.M., Teixeira, A.R., Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis (1996) Rev Inst Med Trop Sao Paulo, 38 (3), pp. 177-185. , http://dx.doi.org/10.1590/S0036-46651996000300003.PMid:9163981