Artículos de revistas
Proteomic And Histopathological Response In The Gills Of Poecilia Reticulata Exposed To Glyphosate-based Herbicide
Registro en:
Environmental Toxicology And Pharmacology. Elsevier, v. 40, n. 1, p. 175 - 186, 2015.
13826689
10.1016/j.etap.2015.04.016
2-s2.0-84933527282
Institución
Resumen
Glyphosate-based herbicides (GBH) are one of the most used herbicide nowadays, whilst there is growing concern over their impact on aquatic environment. Since data about the early proteomic response and toxic mechanisms of GBH in fish is very limited, the aim of this study was to investigate the early toxicity of GBH in the gills of guppies Poecilia reticulata using a proteomic approach associated with histopathological index. Median lethal concentration (LC<inf>50,96h</inf>) was determined and LC<inf>50,96h</inf> values of guppies exposed to GBH were 3.6±0.4mg GLIL-1. Using two-dimensional gel electrophoresis associated with mass spectrometry, 14 proteins regulated by GBH were identified, which are involved in different cell processes, as energy metabolism, regulation and maintenance of cytoskeleton, nucleic acid metabolism and stress response. Guppies exposed to GBH at 1.82mg GLIL-1 showed time-dependent histopathological response in different epithelial and muscle cell types. 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