Insulin Replacement Restores The Vesicular Secretory Apparatus In The Diabetic Rat Lacrimal Gland.

dc.creatorDias, Ana Carolina
dc.creatorBatista, Thiago Martins
dc.creatorRoma, Letícia Prates
dc.creatorMódulo, Carolina Maria
dc.creatorMalki, Leonardo Tannus
dc.creatorDias, Lara Cristina
dc.creatorAlves, Mônica
dc.creatorReinach, Peter Sol
dc.creatorCarneiro, Everardo Magalhães
dc.creatorRocha, Eduardo Melani
dc.date
dc.date2016-05-23T19:41:32Z
dc.date2016-05-23T19:41:32Z
dc.date.accessioned2018-03-29T01:29:02Z
dc.date.available2018-03-29T01:29:02Z
dc.identifierArquivos Brasileiros De Oftalmologia. v. 78, n. 3, p. 158-163
dc.identifier1678-2925
dc.identifier10.5935/0004-2749.20150041
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/?term=26222104
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/235566
dc.identifier26222104
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1303809
dc.descriptionIn the lacrimal gland (LG) acinar cells, signaling regulates the release of secretory vesicles through specific Rab and SNARE exocytotic proteins. In diabetes mellitus (DM), the LGs are dysfunctional. The aim of this work was to determine if secretory apparatus changes were associated with any effects on the secretory vesicles (SV) in diabetic rats as well as the expression levels of constituent Rab and members of the SNARE family, and if insulin supplementation reversed those changes. DM was induced in male Wistar rats with an intravenous dose of streptozotocin (60 mg/kg). One of the two diabetic groups was then treated every other day with insulin (1 IU). A third control group was injected with vehicle. After 10 weeks, Western blotting and RT-PCR were used to compared the Rab and SNARE secretory factor levels in the LGs. Transmission electron microscopy evaluated acinar cell SV density and integrity. In the diabetes mellitus group, there were fewer and enlarged SV. The Rab 27b, Rab 3d, and syntaxin-1 protein expression declined in the rats with diabetes mellitus. Insulin treatment restored the SV density and the Rab 27b and syntaxin expression to their control protein levels, whereas the Vamp 2 mRNA expression increased above the control levels. Diabetes mellitus LG changes were associated with the declines in protein expression levels that were involved in supporting exocytosis and vesicular formation. They were partially reversed by insulin replacement therapy. These findings may help to improve therapeutic management of dry eye in diabetes mellitus.
dc.description78
dc.description158-163
dc.languageeng
dc.relationArquivos Brasileiros De Oftalmologia
dc.relationArq Bras Oftalmol
dc.rightsaberto
dc.sourcePubMed
dc.subjectAcetylcholine
dc.subjectAcinar Cells
dc.subjectAnimals
dc.subjectBlotting, Western
dc.subjectDiabetes Mellitus, Experimental
dc.subjectExocytosis
dc.subjectHypoglycemic Agents
dc.subjectInsulin
dc.subjectLacrimal Apparatus
dc.subjectMale
dc.subjectModels, Animal
dc.subjectQa-snare Proteins
dc.subjectR-snare Proteins
dc.subjectRna, Messenger
dc.subjectRats, Wistar
dc.subjectSecretory Vesicles
dc.subjectRab Gtp-binding Proteins
dc.titleInsulin Replacement Restores The Vesicular Secretory Apparatus In The Diabetic Rat Lacrimal Gland.
dc.typeArtículos de revistas


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