Artículos de revistas
Desenvolvimento e validação de método espectrofotométrico para determinação de corante à base de luteína adicionado em iogurte desnatado
Development and validation of a spectrophotometric method for determination of lutein colorant added to nonfat yogurt
Registro en:
Química Nova. Sociedade Brasileira de Química, v. 35, n. 10, p. 2057-2062, 2012.
0100-4042
S0100-40422012001000028
10.1590/S0100-40422012001000028
Autor
Xavier, Ana Augusta Odorissi
Mercadante, Adriana Zerlotti
Domingos, Lígia Dozena
Viotto, Walkíria Hanada
Institución
Resumen
A simple analytical method for extraction and quantification of lutein colorant added to yogurt was developed and validated. The method allowed complete extraction of carotenoids using tetrahydrofuran in vortex, followed by centrifugation, partition to diethyl ether/petroleum ether, and drying. The carotenoids dissolved in ethanol were quantified by UV-Vis spectrophotometry. This method showed linearity in the range tested (1.41-13.42 µg g-1), limits of detection and quantification of 0.42 and 1.28 µg g-1, respectively, low relative standard deviation (3.4%) and recovery ranging from 95 to 103%. The method proved reliable for quantification of lutein added to yogurt. 2057 2062