Artículos de revistas
Tissue Microarray Is A Reliable Method For Immunohistochemical Analysis Of Pleomorphic Adenoma.
Registro en:
Oral Surgery, Oral Medicine, Oral Pathology And Oral Radiology. v. 117, n. 1, p. 81-8, 2014-Jan.
2212-4411
10.1016/j.oooo.2013.08.029
24332331
Autor
Fonseca, Felipe Paiva
de Andrade, Bruno Augusto Benevenuto
Rangel, Ana Lúcia Carrinho Ayrosa
Della Coletta, Ricardo
Lopes, Márcio Ajudarte
de Almeida, Oslei Paes
Vargas, Pablo Agustin
Institución
Resumen
To determine the most adequate number and size of tissue microarray (TMA) cores for pleomorphic adenoma immunohistochemical studies. Eighty-two pleomorphic adenoma cases were distributed in 3 TMA blocks assembled in triplicate containing 1.0-, 2.0-, and 3.0-mm cores. Immunohistochemical analysis against cytokeratin 7, Ki67, p63, and CD34 were performed and subsequently evaluated with PixelCount, nuclear, and microvessel software applications. The 1.0-mm TMA presented lower results than 2.0- and 3.0-mm TMAs versus conventional whole section slides. Possibly because of an increased amount of stromal tissue, 3.0-mm cores presented a higher microvessel density. Comparing the results obtained with one, two, and three 2.0-mm cores, there was no difference between triplicate or duplicate TMAs and a single-core TMA. Considering the possible loss of cylinders during immunohistochemical reactions, 2.0-mm TMAs in duplicate are a more reliable approach for pleomorphic adenoma immunohistochemical study. 117 81-8