The aim of this study was to evaluate the effect of the prosthesis material (metal-acrylic resin or metal-ceramic) on loosening torque of the prosthetic screws in an implant-supported mandibular denture under two levels of vertical misfit. Ten frameworks were fabricated with commercially pure titanium, and five of them received acrylic resin and acrylic artificial teeth as veneering material and the other five were veneered with porcelain. Two levels of vertical fit were also created by fabricating 20 cast models to obtain four experimental groups according to the prosthesis material and misfit: Group 1 (metal-acrylic resin prosthesis with a passive fit); Group 2 (metal-acrylic resin prosthesis with a non-passive fit); Group 3 (metal-ceramic prosthesis with a passive fit); and Group 4 (metal-ceramic prosthesis with a non-passive fit). Two hundred prosthetic titanium-alloy screws were divided in 40 sets (five screws per set, n=10). After 24h, the loosening torque of the screws was evaluated using a digital torque meter. The results were submitted to two-way ANOVA analysis of variance followed by a Tukey's test (α=0.05). The mean values and standard deviations for each group were G1=7.05 (1.64), G2=5.52 (0.90), G3=6.46 (1.34), and G4=4.35 (0.99). Overall, the prosthesis material and misfit factors showed a statistically significant influence on the loosening torque (p<0.05). Metal-ceramic prosthesis and misfits decreased the loosening of the torque of the prosthetic screws.461358-62