Artículos de revistas
Prilocaine-cyclodextrin-liposome: Effect Of Ph Variations On The Encapsulation And Topology Of A Ternary Complex Using 1h Nmr.
Registro en:
Magnetic Resonance In Chemistry : Mrc. v. 49, n. 6, p. 295-300, 2011-Jun.
1097-458X
10.1002/mrc.2740
21452355
Autor
Cabeça, Luís Fernando
Figueiredo, Isis Martins
de Paula, Eneida
Marsaioli, Anita Jocelyne
Institución
Resumen
A better comprehension of the prilocaine (PLC)-β-cyclodextrin (β-CD) complex liberation to membranes was provided by studying the architectural supramolecular arrangements of PLC, β-CD and egg phosphatidylcholine (EPC) liposomes, a membrane model. The topologies and possible interactions of mixtures of PLC, β-CD and EPC liposomes were investigated by nuclear magnetic resonances combining experimental (1)H-NMR (1D ROESY, STD and DOSY) at different pHs. The results indicate that in the mixture PLC/β-CD/EPC at pH 10 the PLC molecules are almost totally embedded into the liposomes and little interaction was observed between PLC and β-CD. However, at pH 5.5 not only was PLC imbedded in the EPC bilayer, but PLC was also interacting with β-CD. These results were rationalized as a spontaneous PLC release from β-CD to liposomes vesicles, whereas the PLC/EPC complex formation was higher at pH 10 than pH 5.5. 49 295-300