Artículos de revistas
Efficacy Of Pentavalent Antimony, Amphotericin B, And Miltefosine In Leishmania Amazonensis-infected Macrophages Under Normoxic And Hypoxic Conditions.
Registro en:
The Journal Of Parasitology. v. 94, n. 6, p. 1415-7, 2008-Dec.
0022-3395
10.1645/GE-1613.1
18576874
Autor
Ayres, D C
Pinto, L A
Giorgio, S
Institución
Resumen
Recently, our group demonstrated that mouse lesions infected with Leishmania amazonensis are hypoxic. Evidence indicates the negative impact of hypoxia on the efficacy of a variety of chemotherapeutic agents against tumors, fungi, bacteria, and malaria parasites. In the present study, comparison of the effect of antileishmanial drugs on L. amazonensis-infected macrophages under normoxic and hypoxic conditions was performed. We compared the effect of 5% oxygen tension with a tension of 21% oxygen on peritoneal murine macrophage cultures infected with the parasite and treated with glucantime, amphotericin B, or miltefosine. Analysis of the infection index (percentage of infected macrophages x number of amastigotes per macrophage), dose-dependent efficacy of drugs, and IC(50) values demonstrated that hypoxia conferred a small, but significant, resistance to all 3 antileishmanial drugs. The present finding suggests that in vitro assays under hypoxia should not be neglected in drug studies. 94 1415-7