High Susceptibility Of Activated Lymphocytes To Oxidative Stress-induced Cell Death.

dc.creatorDegasperi, Giovanna R
dc.creatorCastilho, Roger F
dc.creatorVercesi, Anibal E
dc.date2008-Mar
dc.date2015-11-27T13:12:36Z
dc.date2015-11-27T13:12:36Z
dc.date.accessioned2018-03-29T01:06:27Z
dc.date.available2018-03-29T01:06:27Z
dc.identifierAnais Da Academia Brasileira De Ciências. v. 80, n. 1, p. 137-48, 2008-Mar.
dc.identifier0001-3765
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/18345382
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197712
dc.identifier18345382
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297945
dc.descriptionThe present study provides evidence that activated spleen lymphocytes from Walker 256 tumor bearing rats are more susceptible than controls to tert-butyl hydroperoxide (t-BOOH)-induced necrotic cell death in vitro. The iron chelator and antioxidant deferoxamine, the intracellular Ca2+ chelator BAPTA, the L-type Ca2+ channel antagonist nifedipine or the mitochondrial permeability transition inhibitor cyclosporin A, but not the calcineurin inhibitor FK-506, render control and activated lymphocytes equally resistant to the toxic effects of t-BOOH. Incubation of activated lymphocytes in the presence of t-BOOH resulted in a cyclosporin A-sensitive decrease in mitochondrial membrane potential. These results indicate that the higher cytosolic Ca2+ level in activated lymphocytes increases their susceptibility to oxidative stress-induced cell death in a mechanism involving the participation of mitochondrial permeability transition.
dc.description80
dc.description137-48
dc.languageeng
dc.relationAnais Da Academia Brasileira De Ciências
dc.relationAn. Acad. Bras. Cienc.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectCalcium
dc.subjectCarcinoma 256, Walker
dc.subjectChelating Agents
dc.subjectDeferoxamine
dc.subjectEgtazic Acid
dc.subjectFlow Cytometry
dc.subjectLymphocyte Activation
dc.subjectLymphocytes
dc.subjectMale
dc.subjectMembrane Potentials
dc.subjectMitochondria
dc.subjectNifedipine
dc.subjectOxidation-reduction
dc.subjectOxidative Stress
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSiderophores
dc.subjectSpleen
dc.subjectTime Factors
dc.subjectTert-butylhydroperoxide
dc.titleHigh Susceptibility Of Activated Lymphocytes To Oxidative Stress-induced Cell Death.
dc.typeArtículos de revistas


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