Artículos de revistas
Analysis Of Adenosine By Rp-hplc Method And Its Application To The Study Of Adenosine Kinase Kinetics.
Registro en:
Journal Of Separation Science. v. 30, n. 15, p. 2473-9, 2007-Oct.
1615-9306
10.1002/jssc.200700194
17763527
Autor
Marin, Rodrigo Miguel
Franchini, Kleber Gomes
Rocco, Silvana Aparecida
Institución
Resumen
An RP-HPLC method for the analysis of adenosine (ADO) has been developed and validated. In the present study, we report an RP-HPLC-based method with modifications of mobile phase and shorter retention time that substantially improved the efficiency of ADO analysis. The HPLC separation of the ADO was achieved on a C18 column, using a mobile phase consisting of water, containing 7% v/v ACN, at a flow rate of 0.8 mL/min. The column effluent was monitored by UV detection at 260 nm. A linear response was achieved over the concentration range of 0.25-100.00 micromol/L. The analytical method inter- and intra-run accuracy and precision were better than +/- 15%. The LOQ was 0.25 micromol/L, with ADO detection in the range of 6.25 pmol per sample. The method has been applied to the study of adenosine kinase (AK) kinetics. 30 2473-9