Identification Of Genes Responsive To The Application Of Ethanol On Sugarcane Leaves.

dc.creatorCamargo, Sandra R
dc.creatorCançado, Geraldo M A
dc.creatorUlian, Eugênio C
dc.creatorMenossi, Marcelo
dc.date2007-Dec
dc.date2015-11-27T13:10:22Z
dc.date2015-11-27T13:10:22Z
dc.date.accessioned2018-03-29T01:05:18Z
dc.date.available2018-03-29T01:05:18Z
dc.identifierPlant Cell Reports. v. 26, n. 12, p. 2119-28, 2007-Dec.
dc.identifier0721-7714
dc.identifier10.1007/s00299-007-0430-8
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17701412
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197417
dc.identifier17701412
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297650
dc.descriptionThe control of gene expression in precise time and space is a desirable attribute of chemically inducible systems. Ethanol is a chemical inducer with favourable features, such as being inexpensive and easy to apply. The aim of this study was to identify ethanol-responsive genes in sugarcane. The cDNA macroarray technique was adopted to identify transcript changes in sugarcane leaves (Saccharum spp. cv SP80-3280) exposed to ethanol. The expression profiles of sugarcane genes were analysed using nylon filters containing 3,575 cDNA clones from the leaf roll library of the SUCEST project. Seventy expressed sequence tags (ESTs) presented altered expression patterns, including ESTs corresponding to genes related to transcriptional and translational processes, abiotic stress and others. Several genes of unknown function were also identified. Among the 48 ESTs up-regulated by ethanol, an abiotic stress-responsive protein and an unknown function gene presented rapid induction by ethanol. The macroarray data of selected ethanol-responsive EST were confirmed by RNA-blot hybridisation. The expression profile of the 48 up-regulated genes was compared in two other cultivars: SP89-1115 and SP90-3414. Surprisingly, no gene showed a similar expression profile in the three cultivars. This result suggests that sugarcane plants have a high diversity in their responses to ethanol.
dc.description26
dc.description2119-28
dc.languageeng
dc.relationPlant Cell Reports
dc.relationPlant Cell Rep.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBlotting, Northern
dc.subjectEthanol
dc.subjectExpressed Sequence Tags
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Plant
dc.subjectGenes, Plant
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectPlant Leaves
dc.subjectPlant Proteins
dc.subjectSaccharum
dc.titleIdentification Of Genes Responsive To The Application Of Ethanol On Sugarcane Leaves.
dc.typeArtículos de revistas


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