Preparation Of Stationary Phases For Reversed-phase High-performance Liquid Chromatography Using Thermal Treatments At High Temperature.

dc.creatorVigna, Camila R M
dc.creatorBottoli, Carla B G
dc.creatorCollins, Kenneth E
dc.creatorCollins, Carol H
dc.date2007-Jul
dc.date2015-11-27T13:10:02Z
dc.date2015-11-27T13:10:02Z
dc.date.accessioned2018-03-29T01:04:43Z
dc.date.available2018-03-29T01:04:43Z
dc.identifierJournal Of Chromatography. A. v. 1156, n. 1-2, p. 60-7, 2007-Jul.
dc.identifier0021-9673
dc.identifier10.1016/j.chroma.2007.04.007
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17462660
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197263
dc.identifier17462660
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297496
dc.descriptionBatches of poly(methyloctylsiloxane) (PMOS)-loaded silica were prepared by deposition from a solution of PMOS into the pores of HPLC silica. Portions of PMOS-loaded silica were subjected to a thermal treatment at 100 degrees C for 24h (condition 1) in a tube furnace under a nitrogen atmosphere. After that, the material was heated for 4h at higher temperatures (150-400 degrees C) (condition 2). Heating at higher temperatures produces polymer bilayers. Non-immobilized and thermally treated stationary phases were characterized by percent carbon, (29)Si cross-polarization magic angle spinning nuclear magnetic resonance spectroscopy and reversed-phase chromatographic performance. The results show that thermal treatment between 150 and 300 degrees C accelerates the immobilization process, possibly due to some bond breaking of the polysiloxane, with formation of strong linkages to the surface of the support, resulting in more complete coverage of the silica. The chromatographic results show an improvement of efficiency with the increase of the temperature of condition 2 up to 300 degrees C and an increase in the resolution of the components, mainly for the phase heated at 300 degrees C. Such results demonstrate that a two-step thermal treatment (100 degrees C then 150-300 degrees C) produces stationary phases with good properties for use in reversed-phase high-performance liquid chromatography.
dc.description1156
dc.description60-7
dc.languageeng
dc.relationJournal Of Chromatography. A
dc.relationJ Chromatogr A
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectChromatography, High Pressure Liquid
dc.subjectHot Temperature
dc.subjectSilicon Dioxide
dc.subjectSiloxanes
dc.titlePreparation Of Stationary Phases For Reversed-phase High-performance Liquid Chromatography Using Thermal Treatments At High Temperature.
dc.typeArtículos de revistas


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