Artículos de revistas
Inducible-nos But Not Neuronal-nos Participate In The Acute Effect Of Tnf-alpha On Hypothalamic Insulin-dependent Inhibition Of Food Intake.
Registro en:
Febs Letters. v. 580, n. 19, p. 4625-31, 2006-Aug.
0014-5793
10.1016/j.febslet.2006.07.042
16876161
Autor
Moraes, Juliana C
Amaral, Maria E
Picardi, Paty K
Calegari, Vivian C
Romanatto, Talita
Bermúdez-Echeverry, Marcela
Chiavegatto, Silvana
Saad, Mario J
Velloso, Licio A
Institución
Resumen
TNF-alpha acts on the hypothalamus modulating food intake and energy expenditure through mechanisms incompletely elucidated. Here, we explore the hypothesis that, to modulate insulin-induced anorexigenic signaling in hypothalamus, TNF-alpha requires the synthesis of NO. TNF-alpha activates signal transduction through JNK and p38 in hypothalamus, peaking at 10(-8) M. This is accompanied by the induction of expression of the inducible and neuronal forms of NOS, in both cases peaking at 10(-12) M. In addition, TNF-alpha stimulates NOS catalytic activity. Pre-treatment with TNF-alpha at a low dose (10(-12) M) inhibits insulin-dependent anorexigenic signaling, and this effect is abolished in iNOS but not in nNOS knockout mice. 580 4625-31