Artículos de revistas
Comparative Analysis Of Two-dimensional Electrophoresis Maps (2-de) Of Helicobacter Pylori From Brazilian Patients With Chronic Gastritis And Duodenal Ulcer: A Preliminary Report.
Registro en:
Revista Do Instituto De Medicina Tropical De São Paulo. v. 48, n. 3, p. 175-7
0036-4665
/S0036-46652006000300011
16847509
Autor
Pereira, Diego R B
Martins, Daniel
Winck, Flavia V
Smolka, Marcus B
Nishimura, Nancy F
Rabelo-Gonçalves, Elizabeth M A
Hara, Natalicia H
Marangoni, Sérgio
Zeitune, José Murilo R
Novello, José Camillo
Institución
Resumen
Helicobacter pylori is a bacterium recognized as the major cause of peptic ulcer and chronic gastritis. Recently, a proteome-based approach was developed to investigate pathogenic factors related to H. pylori. In this preliminary study, H. pylori strains were isolated from gastric biopsies of patients with chronic gastritis and duodenal ulcers. A partial proteomic analysis of H. pylori strains was performed by bacterial lyses and proteins were separated by two-dimensional gel electrophoresis (2-DE). A comparative analysis was performed to verify a differential protein expression between these two 2-DE maps. These data should be useful to clarify the role of different proteins related to bacterial pathogenesis. This study will be completed using a larger number of samples and protein identification of H. pylori by MALDI-TOF mass spectrometry. 48 175-7