Morphological And Growth Alterations In Vero Cells Transformed By Cisplatin.

dc.creatorGonçalves, Estela Maria
dc.creatorVentura, Cláudio Angelo
dc.creatorYano, Tomomasa
dc.creatorRodrigues Macedo, Maria Lígia
dc.creatorGenari, Selma Candelária
dc.date2006-Jun
dc.date2015-11-27T13:05:28Z
dc.date2015-11-27T13:05:28Z
dc.date.accessioned2018-03-29T01:02:51Z
dc.date.available2018-03-29T01:02:51Z
dc.identifierCell Biology International. v. 30, n. 6, p. 485-94, 2006-Jun.
dc.identifier1065-6995
dc.identifier10.1016/j.cellbi.2005.12.007
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/16716608
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196781
dc.identifier16716608
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297014
dc.descriptionCisplatin is an antineoplastic agent used to treat solid tumours, such as ovarian, testicular and bladder tumours. However, studies in vitro and in vivo have shown that cisplatin is mutagenic, genotoxic and tumorigenic in other tissues and organs. In this work, we examined the effect of cisplatin on Vero cells, a fibroblast-like cell line. The morphological characteristics were investigated using phase contrast microscopy, scanning electron microscopy and the actin cytoskeleton was labelled with fluorescein isothiocyanate-phalloidin. Cell proliferation was assessed based on the growth curve. Cultured Vero cells treated with cisplatin showed behavioural and morphological alterations associated with cellular transformation. The transformed cells grew in multilayers and formed cellular aggregates. The proliferation and morphological characteristics of the transformed cells were very different from those of control ones. Since transformed Vero cells showed several characteristics related to neoplastic growth, these cells could be a useful model for studying tumour cells in vitro.
dc.description30
dc.description485-94
dc.languageeng
dc.relationCell Biology International
dc.relationCell Biol. Int.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectActins
dc.subjectAnimals
dc.subjectAntineoplastic Agents
dc.subjectCarcinogenicity Tests
dc.subjectCell Culture Techniques
dc.subjectCell Division
dc.subjectCell Line
dc.subjectCell Shape
dc.subjectCell Transformation, Neoplastic
dc.subjectCercopithecus Aethiops
dc.subjectCisplatin
dc.subjectMicroscopy, Electron, Scanning
dc.subjectMicroscopy, Phase-contrast
dc.subjectVero Cells
dc.titleMorphological And Growth Alterations In Vero Cells Transformed By Cisplatin.
dc.typeArtículos de revistas


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