High Frequency Of Partial Diiia And Dar Alleles Found In Sickle Cell Disease Patients Suggests Increased Risk Of Alloimmunization To Rhd.

dc.creatorCastilho, L
dc.creatorRios, M
dc.creatorRodrigues, A
dc.creatorPellegrino, J
dc.creatorSaad, S T O
dc.creatorCosta, F F
dc.date2005-Feb
dc.date2015-11-27T13:02:48Z
dc.date2015-11-27T13:02:48Z
dc.date.accessioned2018-03-29T01:02:08Z
dc.date.available2018-03-29T01:02:08Z
dc.identifierTransfusion Medicine (oxford, England). v. 15, n. 1, p. 49-55, 2005-Feb.
dc.identifier0958-7578
dc.identifier10.1111/j.1365-3148.2005.00548.x
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15713129
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196594
dc.identifier15713129
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296827
dc.descriptionWe have set out to determine the frequency of DIIIa and DAR alleles among sickle cell disease (SCD) patients. These D variants permit the unexpected development of antibodies to RhD among individuals who are otherwise classified as RhD+. DNA samples from 130 SCD patients were tested for 455A>C (specific for DIIIa), 602C>G, 667T>G (common for both DIIIa and DAR) and 1025T>C (specific for DAR) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequence analysis. The PCR-RFLP showed that 12 (9.2%) of the SCD patients were carrying DIIIa and DAR alleles. Genomic DNA analysis performed by sequence showed that three samples were heterozygous DIIIa (2.3%), seven heterozygous DAR (4.6%) and two (1.5%) samples carried a partial D with four mutations: 455A>C (heterozygous), 602C>G and 667T>G (homozygous) and 1025T>C (heterozygous), indicating compound heterozygosity for one DIIIa allele and one DAR allele. The predicted phenotypes of eight (6.2%) SCD patients were DIIIa, DAR and DIIIa/DAR. Three patients were anti-D immunized (DAR, n = 1; DIIIa/DAR, n = 2). These findings suggest that SCD patients who are candidates for chronic transfusion may benefit from genotyping for DIIIa and DAR to prevent alloimmunization.
dc.description15
dc.description49-55
dc.languageeng
dc.relationTransfusion Medicine (oxford, England)
dc.relationTransfus Med
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAlleles
dc.subjectAnemia, Sickle Cell
dc.subjectBlood Transfusion
dc.subjectDiabetes Mellitus, Type 1
dc.subjectGene Frequency
dc.subjectHumans
dc.subjectImmunization
dc.subjectRh-hr Blood-group System
dc.subjectRisk Factors
dc.titleHigh Frequency Of Partial Diiia And Dar Alleles Found In Sickle Cell Disease Patients Suggests Increased Risk Of Alloimmunization To Rhd.
dc.typeArtículos de revistas


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