High Frequency Of Human Cytomegalovirus Dna In The Liver Of Infants With Extrahepatic Neonatal Cholestasis.

dc.creatorDe Tommaso, Adriana M A
dc.creatorAndrade, Paula D
dc.creatorCosta, Sandra C B
dc.creatorEscanhoela, Cecília A F
dc.creatorHessel, Gabriel
dc.date2005
dc.date2015-11-27T13:02:37Z
dc.date2015-11-27T13:02:37Z
dc.date.accessioned2018-03-29T01:01:47Z
dc.date.available2018-03-29T01:01:47Z
dc.identifierBmc Infectious Diseases. v. 5, p. 108, 2005.
dc.identifier1471-2334
dc.identifier10.1186/1471-2334-5-108
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/16321152
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196505
dc.identifier16321152
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296738
dc.descriptionBiliary atresia (BA) is the most severe hepatic disorder in newborns and its etiopathogenesis remains unknown. Viral involvement has been proposed, including the human cytomegalovirus (HCMV). The aims of the study were to use the polymerase chain reaction (PCR) to screen the liver tissue of infants with extrahepatic cholestasis for HCMV and to correlate the results with serological antibodies against HCMV and histological findings. A retrospective study in a tertiary care setting included 35 patients (31 BA, 1 BA associated with a choledochal cyst, 2 congenital stenosis of the distal common bile duct and 1 hepatic cyst). HCMV serology was determined by ELISA. Liver and porta hepatis were examined histologically. Liver samples from infants and a control group were screened for HCMV DNA. Twelve patients had HCMV negative serology, 9 were positive for IgG antibodies and 14 were positive for IgG and IgM. Nine liver and seven porta hepatis samples were positive for HCMV DNA but none of the control group were positive (general frequency of positivity was 34.3%-12/35). There was no correlation between HCMV positivity by PCR and the histological findings. The accuracy of serology for detecting HCMV antibodies was low. These results indicate an elevated frequency of HCMV in pediatric patients with extrahepatic neonatal cholestasis. They also show the low accuracy of serological tests for detecting active HCMV infection and the lack of correlation between HCMV positivity by PCR and the histopathological changes.
dc.description5
dc.description108
dc.languageeng
dc.relationBmc Infectious Diseases
dc.relationBMC Infect. Dis.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAntibodies, Viral
dc.subjectBiliary Atresia
dc.subjectCholedochal Cyst
dc.subjectCholestasis, Extrahepatic
dc.subjectCytomegalovirus
dc.subjectDna, Viral
dc.subjectFemale
dc.subjectHumans
dc.subjectImmunoglobulin G
dc.subjectImmunoglobulin M
dc.subjectInfant
dc.subjectLiver
dc.subjectMale
dc.subjectRetrospective Studies
dc.titleHigh Frequency Of Human Cytomegalovirus Dna In The Liver Of Infants With Extrahepatic Neonatal Cholestasis.
dc.typeArtículos de revistas


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