Metal-binding Proteins Scanning And Determination By Combining Gel Electrophoresis, Synchrotron Radiation X-ray Fluorescence And Atomic Spectrometry.

dc.creatorVerbi, F M
dc.creatorArruda, S C C
dc.creatorRodriguez, A P M
dc.creatorPérez, C A
dc.creatorArruda, M A Z
dc.date2005-Feb
dc.date2015-11-27T13:02:36Z
dc.date2015-11-27T13:02:36Z
dc.date.accessioned2018-03-29T01:01:46Z
dc.date.available2018-03-29T01:01:46Z
dc.identifierJournal Of Biochemical And Biophysical Methods. v. 62, n. 2, p. 97-109, 2005-Feb.
dc.identifier0165-022X
dc.identifier10.1016/j.jbbm.2004.09.008
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15680280
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196502
dc.identifier15680280
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296735
dc.descriptionIn the present work, protein bands from in vitro embriogenic callus (Citrus sinensis L. Osbeck) were investigated using micro-synchrotron radiation X-ray fluorescence (muSR-XRF) after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separation. Metal-binding protein quantification was done after microwave oven decomposition of gel by synchrotron radiation total reflection X-ray fluorescence (SR-TXRF), flame atomic absorption spectrometry (FAAS) and flame atomic emission spectrometry (FAES). According to the analysis of the protein bands, it is possible to observe that both 81 and ca. 14 kDa proteins present different Fe signal intensity at different positions. The analysis of 53 kDa protein, showed even more interesting results. Besides Fe, the muSR-XRF experiments indicate the presence of Ca, Cu, K and Zn. Chemical elements such as Cu, K, Fe and Zn were determined by SR-TXRF, Mg by FAAS and Na by FAES. Ca was determined by SR-TXRF and FAAS only for accuracy check. In the mineralised protein bands of 81 and around 14 kDa band, only Fe was determined (105 and 21.8 microg g(-1)). For those protein bands (86-ca. 14 kDa) were determined, Ca, K, Cu and Zn in a wide concentration range (42.4-283, 2.47-96.8, 0.91-15.9 and 3.39-29.7 microg g(-1), respectively).
dc.description62
dc.description97-109
dc.languageeng
dc.relationJournal Of Biochemical And Biophysical Methods
dc.relationJ. Biochem. Biophys. Methods
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBiophysics
dc.subjectCalcium
dc.subjectCitrus
dc.subjectCopper
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectMetals
dc.subjectMolecular Weight
dc.subjectPotassium
dc.subjectProtein Binding
dc.subjectProteins
dc.subjectSodium
dc.subjectSpectrometry, Fluorescence
dc.subjectSpectrophotometry, Atomic
dc.subjectSynchrotrons
dc.subjectTemperature
dc.subjectX-rays
dc.subjectZinc
dc.titleMetal-binding Proteins Scanning And Determination By Combining Gel Electrophoresis, Synchrotron Radiation X-ray Fluorescence And Atomic Spectrometry.
dc.typeArtículos de revistas


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