Co-expression And Regulation Of Connexins 36 And 43 In Cultured Neonatal Rat Pancreatic Islets.

dc.creatorLeite, A R
dc.creatorCarvalho, C P F
dc.creatorFurtado, A G
dc.creatorBarbosa, H C L
dc.creatorBoschero, A C
dc.creatorCollares-Buzato, C B
dc.date2005-Feb
dc.date2015-11-27T13:01:58Z
dc.date2015-11-27T13:01:58Z
dc.date.accessioned2018-03-29T01:00:35Z
dc.date.available2018-03-29T01:00:35Z
dc.identifierCanadian Journal Of Physiology And Pharmacology. v. 83, n. 2, p. 142-51, 2005-Feb.
dc.identifier0008-4212
dc.identifier10.1139/y04-133
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15791287
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196202
dc.identifier15791287
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296435
dc.descriptionFetal and neonatal pancreatic islets present a lower insulin secretory response as compared with adult islets. Prolonged culturing leads to an improvement of the glucose-induced insulin secretion response in neonatal pancreatic islets that may involve regulation of gap junction mediated cell communication. In this study, we investigated the effect of culturing neonatal islet cells for varying periods of time and with different glucose medium concentrations on the cellular expression of the endocrine pancreatic gap junction associated connexin (Cx) 36 and Cx43. We report here that the 7-d culture induced upregulation of the expression of these junctional proteins in neonatal islets in a time-dependent manner. A correlation was observed between the increased mRNA and protein expression of Cx36 and Cx43 and the increased insulin secretion following islet culturing. In addition, increasing glucose concentration within the culture medium induced a concentration-dependent enhancement of Cx36 islet expression, but not of Cx43 expression in cultured neonatal islets. In conclusion, we suggest that the regulation of gap junctional proteins by culture medium containing factors and glucose may be an important event for the maturation process of beta cells observed at in vitro conditions.
dc.description83
dc.description142-51
dc.languageeng
dc.relationCanadian Journal Of Physiology And Pharmacology
dc.relationCan. J. Physiol. Pharmacol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAnimals, Newborn
dc.subjectCell Culture Techniques
dc.subjectCells, Cultured
dc.subjectConnexin 43
dc.subjectConnexins
dc.subjectGlucose
dc.subjectInsulin
dc.subjectIslets Of Langerhans
dc.subjectRna, Messenger
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectTime Factors
dc.subjectUp-regulation
dc.titleCo-expression And Regulation Of Connexins 36 And 43 In Cultured Neonatal Rat Pancreatic Islets.
dc.typeArtículos de revistas


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