Regulation Of The Htpx Gene Of Xylella Fastidiosa And Its Expression In E. Coli.

dc.creatorColtri, Patricia P
dc.creatorRosato, Yoko B
dc.date2004-Jun
dc.date2015-11-27T12:58:11Z
dc.date2015-11-27T12:58:11Z
dc.date.accessioned2018-03-29T00:59:05Z
dc.date.available2018-03-29T00:59:05Z
dc.identifierCurrent Microbiology. v. 48, n. 6, p. 391-5, 2004-Jun.
dc.identifier0343-8651
dc.identifier10.1007/s00284-003-4179-1
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15170231
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195815
dc.identifier15170231
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296048
dc.descriptionXylella fastidiosa was the first phytopathogen to be completely sequenced, and its genome revealed several interesting features to be used in functional studies. In the present work, the htpX gene, which encodes a protein involved in the heat shock response in other bacteria, was analyzed by RT-PCR by using cells derived from different cultural conditions. This gene was induced after a temperature upshift to 37 degrees C after growth in minimal medium, XDM, but showed constitutive expression in rich medium or in XDM plus plant extracts. Sequences upstream to the htpX gene, containing a putative regulatory region, were also transferred to E. coli, and the thermoregulation was maintained in the new host, since it was constitutively transcribed at 37 degrees C or 45 degrees C in all culture media tested, but not at 28 degrees C in minimal culture medium. The gene was also cloned into the expression vector pET32Xa/LIC, and the expression of the corresponding protein was confirmed by Western blotting.
dc.description48
dc.description391-5
dc.languageeng
dc.relationCurrent Microbiology
dc.relationCurr. Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBacterial Proteins
dc.subjectCloning, Molecular
dc.subjectCulture Media
dc.subjectEscherichia Coli
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenes, Bacterial
dc.subjectHeat-shock Proteins
dc.subjectHeat-shock Response
dc.subjectRna, Bacterial
dc.subjectRna, Messenger
dc.subjectRecombinant Proteins
dc.subjectRegulatory Sequences, Nucleic Acid
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectXylella
dc.titleRegulation Of The Htpx Gene Of Xylella Fastidiosa And Its Expression In E. Coli.
dc.typeArtículos de revistas


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