Artículos de revistas
Lipid Membrane With Low Proton Permeability.
Biochimica Et Biophysica Acta. v. 1611, n. 1-2, p. 1-4, 2003-Apr.
Biloti, Débora Nakai
Santana, Maria Helena Andrade
Pessine, Francisco Benedito Teixeira
This work reports the production of a liposomal formulation, having a lipidic membrane with known chemical composition and a low proton permeability, as confirmed by physicochemical characterization of the maintenance of a transmembranic pH gradient. These liposomes consist of DSPC, DSPE-PEG, DSPG and cholesterol, with low internal pH. To verify the low proton permeability of these liposomal bilayers, a study of proton migration according to the fluorescence quenching of 9-aminoacridine (9AA), as well as CPT-11 encapsulation, were used to monitor the acidification of the intravesicular space. Both experiments showed that this liposomal formulation is able to maintain a transmembranic proton gradient.16111-4